Scopus
🔓 Açık Erişim
Antibacterial activity of seed aqueous extract of Citrus limon (L.) mediated synthesis ZnO NPs: An impact on Zebrafish (Danio rerio) caudal fin development
Heliyon · Eylül 2022
Makale Bilgileri
DergiHeliyon
Yayın TarihiEylül 2022
Cilt / Sayfa8
Scopus ID2-s2.0-85138795050
Erişim🔓 Açık Erişim
Özet
Among the different metal oxide nanoparticles, zinc oxide nanoparticles have gained significant importance due to their antibacterial properties against clinically pathogenic bacteria during the organal development. In the present study, biogenic zinc oxide nanoparticles were synthesized using seed extract of Citrus limon by a simple, cost-effective, and green chemistry approach. The synthesized ZnO NPs were characterized by UV-Vis spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, Dynamic Light Scattering, and Scanning Electron Microscopy. Next, the antimicrobial activity of ZnO NPs was tested against clinically pathogenic bacteria, i.e., Pseudomonas fluorescens, Escherichia coli, Enterobacter aerogenes, and Bacillus subtilis. Followed by, ZnO NPs were evaluated for the development of caudal fin in Zebrafish. The UV-Vis spectram result showed a band at 380 nm and FTIR results confirmed the ZnO NPs. The average crystallite size of the ZnO NPs was 52.65 ± 0.5 nm by the Debye Scherrer equation and SEM showed spherical-shaped particles. A zone of inhibition around ZnO NPs applied to P. fluorescens indicates sensitive to ZnO NPs followed by B. subtilis. Among the four different bacterial pathogens, E. aerogenes was the most susceptible compared to the other three pathogens. The calculated sub-lethal concentration of ZnO NPs at 96 h was 153.8 mg/L with a 95% confidence limit ranging from 70.62 to 214.18 mg/L, which was used with partially amputated zebrafish caudal fin growth. A significant (p < 0.5) development (95%) in the amputated caudal fin was detected at 12 days post-amputation. Low concentrated ZnO NPs can reduce developmental malformation. Collectively, suggested results strongly proved that lemon seed-mediated synthesized ZnO NPs had a good pathogenic barrier for bacterial infection during the external organal development for the first time.
Yazarlar (12)
1
Selvakumar Sakthivel
ORCID: 0000-0002-0953-4426
2
Anand Raj Dhanapal
3
Lilly Pushpa Paulraj
4
Annadurai Gurusamy
5
Baskar Venkidasamy
ORCID: 0000-0002-3888-7079
6
Muthu Thiruvengadam
7
Rajakumar Govindasamy
8
Mohammad Ali Shariati
ORCID: 0000-0001-9376-5771
9
Abdelhakim Bouyahya
ORCID: 0000-0001-9317-1631
10
Gokhan Zengin
ORCID: 0000-0001-6548-7823
11
Mohammad Mehedi Hasan
ORCID: 0000-0002-3871-889X
12
Pavel Burkov
ORCID: 0000-0001-7515-5670
Anahtar Kelimeler
Antibacterial activity
Caudal fin development
Citrus limon seed
Green synthesis
Zebrafish
Zinc oxide nanoparticles
Kurumlar
College of Sanghuh Life Sciences
Seoul South Korea
Faculté des Sciences Rabat
Rabat Morocco
Karpagam Academy of Higher Education
Coimbatore India
Manonmaniam Sundaranar University
Tirunelveli India
Mawlana Bhashani Science and Technology University
Tangail Bangladesh
Moscow State University of Technologies and Management
Moscow Russian Federation
Saveetha Dental College And Hospitals
Chennai India
Selçuk Üniversitesi
Selçuklu Turkey
South Ural State Agrarian University
Troitsk Russian Federation
Metrikler
15
Atıf
12
Yazar
6
Anahtar Kelime