Scopus
🔓 Açık Erişim
Protective Effects of PollenAid Plus Soft Gel Capsules’ Hydroalcoholic Extract in Isolated Prostates and Ovaries Exposed to Lipopolysaccharide
Molecules · Ekim 2022
Makale Bilgileri
DergiMolecules
Yayın TarihiEkim 2022
Cilt / Sayfa27
Scopus ID2-s2.0-85139915454
Erişim🔓 Açık Erişim
Özet
Pollen extract represents an innovative approach for the management of the clinical symptoms related to prostatitis and pelvic inflammatory disease (PID). In this context, the aims of the present work were to analyze the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules, and to evaluate the extract’s cytotoxic effects, in human prostate cancer PC3 cells and human ovary cancer OVCAR-3 cells. Additionally, protective effects were investigated in isolated prostate and ovary specimens exposed to lipopolysaccharide (LPS). The phytochemical investigation identified catechin, chlorogenic acid, gentisic acid, and 3-hydroxytyrosol as the prominent phenolics. The extract did not exert a relevant cytotoxic effect on PC3 and OVCAR-3 cells. However, the extract showed a dose-dependent inhibition of pro-inflammatory IL-6 and TNF-α gene expression in prostate and ovary specimens, and the extract was effective in preventing the LPS-induced upregulation of CAT and SOD gene expression, which are deeply involved in tissue antioxidant defense systems. Finally, a docking approach suggested the capability of catechin and chlorogenic acid to interact with the TRPV1 receptor, playing a master role in prostate inflammation. Overall, the present findings demonstrated anti-inflammatory and antioxidant effects of this formulation; thus, suggesting its capability in the management of the clinical symptoms related to prostatitis and PID.
Yazarlar (15)
1
Annalisa Chiavaroli
ORCID: 0000-0002-3399-967X
2
Simonetta Cristina Di Simone
ORCID: 0000-0001-5041-3848
3
Alessandra Acquaviva
4
Maria Loreta Libero
ORCID: 0000-0002-8133-5691
5
Claudia Campana
6
Lucia Recinella
7
Sheila Leone
8
Luigi Brunetti
9
Giustino Orlando
10
Nilofar
11
Irene Vitale
12
Stefania Cesa
ORCID: 0000-0002-8649-0017
13
Gokhan Zengin
ORCID: 0000-0001-6548-7823
14
Luigi Menghini
ORCID: 0000-0002-7346-7395
15
Claudio Ferrante
Anahtar Kelimeler
gene expression
Graminex pollen
hydroalcoholic extract
inflammation
oxidative stress
phenolic compounds
TRPV1
Kurumlar
Sapienza Università di Roma
Rome Italy
Selçuk Üniversitesi
Selçuklu Turkey
University of G. d'Annunzio Chieti and Pescara
Chieti Italy
Metrikler
6
Atıf
15
Yazar
7
Anahtar Kelime