Scopus
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Chemical Composition and Antioxidant, Antimicrobial, and Anti-Inflammatory Properties of Origanum compactum Benth Essential Oils from Two Regions: In Vitro and In Vivo Evidence and In Silico Molecular Investigations
Molecules · Kasım 2022
Makale Bilgileri
DergiMolecules
Yayın TarihiKasım 2022
Cilt / Sayfa27
Scopus ID2-s2.0-85141565994
Erişim🔓 Açık Erişim
Özet
The purposes of this investigatory study were to determine the chemical composition of the essential oils (EOs) of Origanum compactum from two Moroccan regions (Boulemane and Taounate), as well as the evaluation of their biological effects. Determining EOs’ chemical composition was performed by a gas chromatography–mass spectrophotometer (GC-MS). The antioxidant activity of EOs was evaluated using free radical scavenging ability (DPPH method), fluorescence recovery after photobleaching (FRAP), and lipid peroxidation inhibition assays. The anti-inflammatory effect was assessed in vitro using the 5-lipoxygenase (5-LOX) inhibition test and in vivo using the carrageenan-induced paw edema model. Finally, the antibacterial effect was evaluated against several strains using the disk-diffusion assay and the micro-dilution method. The chemical constituent of O. compactum EO (OCEO) from the Boulemane zone is dominated by carvacrol (45.80%), thymol (18.86%), and α-pinene (13.43%). However, OCEO from the Taounate zone is rich in 3-carene (19.56%), thymol (12.98%), and o-cymene (11.16%). OCEO from Taounate showed higher antioxidant activity than EO from Boulemane. Nevertheless, EO from Boulemane considerably inhibited 5-LOX (IC50 = 0.68 ± 0.02 µg/mL) compared to EO from Taounate (IC50 = 1.33 ± 0.01 µg/mL). A similar result was obtained for tyrosinase inhibition with Boulemane EO and Taounate EO, which gave IC50s of 27.51 ± 0.03 μg/mL and 41.83 ± 0.01 μg/mL, respectively. The in vivo anti-inflammatory test showed promising effects; both EOs inhibit and reduce inflammation in mice. For antibacterial activity, both EOs were found to be significantly active against all strains tested in the disk-diffusion test, but O. compactum EO from the Boulemane region showed the highest activity. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) for O. compactum EO from the Boulemane region ranged from 0.06 to 0.25% (v/v) and from 0.15 to 0.21% (v/v) for O. compactum from the Taounate region. The MBC/MIC index revealed that both EOs exhibited remarkable bactericidal effects.
Yazarlar (16)
1
Samiah Hamad Al-Mijalli
2
Nidal Naceiri Mrabti
3
Hayat Ouassou
4
Ryan A. Sheikh
ORCID: 0000-0003-3275-0861
5
Hamza Assaggaf
ORCID: 0000-0001-5563-9491
6
Saad Bakrim
7
Emad M. Abdallah
8
Mohammed Merae Alshahrani
ORCID: 0000-0002-9387-5492
9
Ahmed Abdullah Al Awadh
ORCID: 0000-0001-5860-530X
10
Learn Han Lee
11
Yusra AlDhaheri
12
Amirhossein Sahebkar
13
Gokhan Zengin
ORCID: 0000-0001-6548-7823
14
Ammar A. Attar
15
Abdelhakim Bouyahya
ORCID: 0000-0001-9317-1631
16
Hanae Naceiri Mrabti
Anahtar Kelimeler
antibacterial action
anti-inflammatory
essential oils
Origanum compactum
tyrosinase
Kurumlar
Faculté de Médecine et de Pharmacie de Rabat
Rabat Morocco
Faculté des Sciences Dhar El Mahraz, Université Sidi Mohamed Ben Abdellah
Fez Morocco
Faculté des Sciences d’Oujda
Oujda Morocco
Faculté des Sciences Rabat
Rabat Morocco
Faculty of Pharmaceutical and Biological Sciences
Lille France
King Abdulaziz University
Jeddah Saudi Arabia
Mashhad University of Medical Sciences
Mashhad Iran
Monash University Malaysia
Bandar Sunway Malaysia
Najran University
Najran Saudi Arabia
Princess Nourah Bint Abdulrahman University
Riyadh Saudi Arabia
Qassim University
Al-Mulida Saudi Arabia
School of Pharmacy
Mashhad Iran
Selçuk Üniversitesi
Selçuklu Turkey
Umm Al-Qura University
Makkah Saudi Arabia
United Arab Emirates University
Al Ain United Arab Emirates
Université Ibn Zohr
Agadir Morocco
Metrikler
6
Atıf
16
Yazar
5
Anahtar Kelime