Scopus
🔓 Açık Erişim
A Study on Chemical Characterization and Biological Abilities of Alstonia boonei Extracts Obtained by Different Techniques
Antioxidants · Kasım 2022
Makale Bilgileri
DergiAntioxidants
Yayın TarihiKasım 2022
Cilt / Sayfa11
Scopus ID2-s2.0-85143848755
Erişim🔓 Açık Erişim
Özet
In the quest for novel therapeutic agents from plants, the choice of extraction solvent and technique plays a key role. In this study, the possible differences in the phytochemical profile and bioactivity (antioxidant and enzyme inhibitory activity) of the Alstonia boonei leaves and stem bark extracted using water, ethyl acetate and methanol, and different techniques, namely infusion, maceration and Soxhlet extraction, were investigated. Data collected showed that methanol extracts of both A. boonei leaves (48.34–53.08 mg gallic acid equivalent [GAE]/g dry extract) and stem bark (37.08–45.72 mg GAE/g dry extract) possessed higher phenolic content compared to the ethyl acetate extracts (leaves: 30.64–40.19 mg GAE/g; stem bark: 34.25–35.64 mg GAE/g). The methanol extracts of A. boonei leaves showed higher radical scavenging and reducing capacity, and these findings were in accordance with phenolic content results. In general, water extracts of A. boonei leaves and stem bark obtained by infusion were poor inhibitors of acetylcholinesterase, α-amylase, α-glucosidase, and tyrosinase, except for butyrylcholinesterase. The chemical profiles of the extracts were determined by UHPLC–MS and the presence of several compounds, such as phenolic acids (caffeic, chlorogenic and ferulic acids, etc.), flavonoids (rutin and isoquercetin) and flavonolignans (Cinchonain isomers). Cell viability was tested using the human peripheral blood monocytic cell line (THP-1), and the extracts were safe up to 25 μg/mL. In addition, anti-inflammatory effects were investigated with the releasing of IL-6 TNF-α and IL-1β. In particular, stem bark extracts exhibited significant anti-inflammatory effects. Data presented in this study highlight the key role of solvent choice in the extraction of bioactive secondary metabolites from plants. In addition, this study appraises the antioxidant and enzyme inhibitory action of A. boonei leaves and stem bark, which are extensively used in traditional medicine.
Yazarlar (12)
1
Adriano Mollica
2
Gokhan Zengin
ORCID: 0000-0001-6548-7823
3
Kouadio Ibrahime Sinan
4
Marcella Marletta
5
Stefano Pieretti
6
Azzurra Stefanucci
7
Ouattara Katinan Etienne
8
József Jekő
9
Zoltán Cziáky
10
Mir Babak Bahadori
11
Carene Marie Nancy Picot-Allain
12
Mohamad Fawzi Mahomoodally
Anahtar Kelimeler
Alstonia
anti-inflammatory
extraction methods
natural agents
phenolics
Kurumlar
Istituto Superiore Di Sanita
Rome Italy
Maragheh University of Medical Sciences
Maragheh Iran
North-West University
Potchefstroom South Africa
Saveetha Dental College And Hospitals
Chennai India
Selçuk Üniversitesi
Selçuklu Turkey
Universita degli Studi Guglielmo Marconi
Rome Italy
Université de Cocody-Abidjan
Abidjan Cote d'Ivoire
University of G. d'Annunzio Chieti and Pescara
Chieti Italy
University of Mauritius
Reduit Mauritius
University of Nyíregyháza
Nyíregyháza Hungary
Metrikler
17
Atıf
12
Yazar
5
Anahtar Kelime