Scopus
Cytotoxicity, Phytochemical Screening and Genetic analysis of Ginger (Zingiber officinale Rosc.) Callus and Rhizome
South African Journal of Botany · Aralık 2022
Makale Bilgileri
DergiSouth African Journal of Botany
Yayın TarihiAralık 2022
Cilt / Sayfa151 · 54-59
Scopus ID2-s2.0-85120034006
Özet
Ginger (Zingiber officinale Rosc.) is well known as distinctive spice with considered therapeutic values. In the current work we aimed to investigate cytotoxic effect, phytochemical screening and genetic analysis of ginger callus and their rhizome. Cytotoxicity of ginger rhizome and callus extracts was examined against some human cancer cell lines HT29, HT116 and MCF-7 by MTT method. Phytochemicals were screened by thin layer chromatography (TLC) and genetic analysis was performed by ISSR markers. Ginger rhizome principle components, 6-gingerol and 6-shogoal were used as standards to detect their presence in callus. Results showed that, rhizome extracts displayed a strong cytotoxicity towards all tested cancer cells with the highest activity (IC50 20.4 ± 3.0 µg/mL; P<0.05) against HT29. IC50 of ginger callus extracts were >100 µg/mL in all examined cancer cell lines. TLC chromatograms of rhizome and callus extracts revealed the presence of phenols and terpenes, whereas 6-gingerol and 6-shogaol were only detected in rhizome extracts. Genetic analysis by ISSR markers revealed occurrence some genetic changes in DNA of callus compared to that of rhizome with total polymorphism 23.07%. In conclusion, ginger cytotoxicity to HT29, HT116 and MCF-7 cancer cells was mainly associated with the presence of 6-shogaol and 6-gingerol in extracts, which appeared as strong cytotoxic effect in rhizome compared to non-cytotoxic effect was evaluated in the callus extracts. Therefore, further studies on the callus elicitation and precursors feeding to induce biosynthesis of ginger bioactive molecules are recommended.
Yazarlar (7)
1
Ammar MA Ali
2
Mawahib EM El-Nour
3
Sakina Yagi
4
Ahmed A. Qahtan
5
Abdurrahman A. Alatar
6
Eslam M. Abdel-Salam
7
Gokhan Zengin
ORCID: 0000-0001-6548-7823
Anahtar Kelimeler
Callus
Cytotoxicity
ISSR primers
Phytochemical
Zingiber officinale
Kurumlar
Al Neelain University
Khartoum Sudan
College of Sciences
Riyadh Saudi Arabia
Hajji University
Hajjah Yemen
Khartoum University
Khartoum Sudan
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
8
Atıf
7
Yazar
5
Anahtar Kelime