Scopus
🔓 Açık Erişim
A grape (Vitis vinifera L.) pomace water extract modulates inflammatory and immune response in SW-480 cells and isolated mouse colon
Phytotherapy Research · Aralık 2022
Makale Bilgileri
DergiPhytotherapy Research
Yayın TarihiAralık 2022
Cilt / Sayfa36 · 4620-4630
Scopus ID2-s2.0-85139194425
Erişim🔓 Açık Erişim
Özet
Grape (Vitis vinifera L.) pomace is a residue derived from the winemaking process, which contains bioactive compounds displaying noteworthy health-promoting properties. The aim of the present study was to investigate the phenolic composition and protective effects of a water extract of grape pomace (WEGP) in colorectal cancer cell line SW480 and in isolated mouse colon exposed to Escherichia coli lipopolysaccharide (LPS). The extract decreased SW-480 cell viability, as well as vascular endothelial factor A (VEGFA), hypoxia-induced factor 1α (HIF1α), and transient receptor potential M8 (TRPM8) LPS-induced gene expression. Moreover, the extract inhibited mRNA levels of nuclear factor kB (NFkB), cyclooxygenase (COX)-2, tumor necrosis factor (TNF)α, interleukin (IL)-6, IL-1β, IL-10, inducible nitric oxide synthase (iNOS), and interferon (IFN)γ, in isolated colon. Conversely, WEGP increased the gene expression of antioxidant catalase (CAT) and superoxide dismutase (SOD), in the same model. The modulatory effects exerted by WEGP could be related, at least in part, to the phenolic composition, with particular regards to the catechin level. Docking calculations also predicted the interactions of catechin toward TRPM8 receptor, deeply involved in colon cancer; thus further suggesting the grape pomace as a valuable source of bioactive extracts and phytochemicals with protective effects in the colon.
Yazarlar (15)
1
Lucia Recinella
2
Annalisa Chiavaroli
ORCID: 0000-0002-3399-967X
3
Serena Veschi
4
Alessandro Cama
5
Alessandra Acquaviva
6
Maria Loreta Libero
ORCID: 0000-0002-8133-5691
7
Sheila Leone
8
Simonetta Cristina Di Simone
ORCID: 0000-0001-5041-3848
9
Ester Pagano
ORCID: 0000-0003-2872-1734
10
Gokhan Zengin
ORCID: 0000-0001-6548-7823
11
Luigi Menghini
ORCID: 0000-0002-7346-7395
12
Luigi Brunetti
13
Angelo Antonio Izzo
14
Giustino Orlando
15
Claudio Ferrante
Anahtar Kelimeler
catechin
colon cancer
grape pomace
inflammation
TRPM8
Vitis vinifera
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Università degli Studi di Napoli Federico II, Facoltà di Medicina e Chirurgia
Naples Italy
University of G. d'Annunzio Chieti and Pescara
Chieti Italy
Veridia Italia Srl
Pescara Italy
Metrikler
11
Atıf
15
Yazar
6
Anahtar Kelime