Scopus
🔓 Açık Erişim
Chemical Constituents, Antioxidant, and Enzyme Inhibitory Activities Supported by In-Silico Study of n-Hexane Extract and Essential Oil of Guava Leaves
Molecules · Aralık 2022
Makale Bilgileri
DergiMolecules
Yayın TarihiAralık 2022
Cilt / Sayfa27
Scopus ID2-s2.0-85144573293
Erişim🔓 Açık Erişim
Özet
Psidium guajava (Guava tree) is one of the most widely known species in the family Myrtaceae. The Guava tree has been reported for its potential antioxidant, anti-inflammatory, antimicrobial, and cytotoxic activities. In the current study, the chemical compositions of the n-hexane extract and the essential oil of P. guajava were investigated using the GC/MS analysis, along with an evaluation of their antioxidant potential, and an investigation into the enzyme inhibition of acetylcholinesterase (AChE), butyrylcholinesterase (BchE), tyrosinase, α-amylase, and α-glucosidase. Moreover, molecular docking of the major identified active sites of the target enzymes were investigated. The chemical characterization of the n-hexane extract and essential oil revealed that squalene (9.76%), α-tocopherol (8.53%), and γ-sitosterol (3.90%) are the major compounds in the n-hexane extract. In contrast, the major constituents of the essential oil are D-limonene (36.68%) and viridiflorol (9.68%). The n-hexane extract showed more antioxidant potential in the cupric reducing antioxidant capacity (CUPRAC), the ferric reducing power (FRAP), and the metal chelating ability (MCA) assays, equivalent to 70.80 ± 1.46 mg TE/g, 26.01 ± 0.97 mg TE/g, and 24.83 ± 0.35 mg EDTAE/g, respectively. In the phosphomolybdenum (PM) assay, the essential oil showed more antioxidant activity equivalent to 2.58 ± 0.14 mmol TE/g. The essential oil demonstrated a potent BChE and tyrosinase inhibitory ability at 6.85 ± 0.03 mg GALAE/g and 61.70 ± 3.21 mg KAE/g, respectively. The α-amylase, and α-glucosidase inhibitory activity of the n-hexane extract and the essential oil varied from 0.52 to 1.49 mmol ACAE/g. Additionally, the molecular docking study revealed that the major compounds achieved acceptable binding scores upon docking with the tested enzymes. Consequently, the P. guajava n-hexane extract and oil can be used as a promising candidate for the development of novel treatment strategies for oxidative stress, neurodegeneration, and diabetes mellitus diseases.
Yazarlar (8)
1
Shaza H. Aly
2
Omayma A. Eldahshan
ORCID: 0000-0002-0972-0560
3
Sara T. Al-Rashood
4
Faizah A. Binjubair
5
Mahmoud A. El Hassab
ORCID: 0000-0002-6795-4816
6
Wagdy M. Eldehna
ORCID: 0000-0001-6996-4017
7
Stefano Dall'Acqua
8
Gokhan Zengin
ORCID: 0000-0001-6548-7823
Anahtar Kelimeler
antioxidants
cholinesterase
enzyme inhibition
GC/MS
Myrtaceae
Psidium guajava
tyrosinase
Kurumlar
Ain Shams University
Cairo Egypt
College of Pharmacy
Riyadh Saudi Arabia
Faculty of Pharmacy
Kafr el-Sheikh Egypt
Faculty of Pharmacy - Ain Shams University
Cairo Egypt
Field of Pharmacy
Ras Sedr Egypt
School of Biotechnology
Badr Egypt
School of Pharmacy
Badr Egypt
Selçuk Üniversitesi
Selçuklu Turkey
Università degli Studi di Padova
Padua Italy
Metrikler
17
Atıf
8
Yazar
7
Anahtar Kelime