Scopus
🔓 Açık Erişim
A Bioengineered Quercetin-Loaded 3D Bio-Polymeric Graft for Tissue Regeneration and Repair
Biomedicines · Aralık 2022
Makale Bilgileri
DergiBiomedicines
Yayın TarihiAralık 2022
Cilt / Sayfa10
Scopus ID2-s2.0-85144948649
Erişim🔓 Açık Erişim
Özet
Phytochemicals extracted from plant sources have potential remedial effects to cure a broad range of acute to severe illnesses and ailments. Quercetin is a flavonoid isolated from different dietary sources such as vegetables and fruits, exhibiting strong anti-inflammatory, anti-oxidative and non-toxic effects on the biological system. However, the direct uptake or administration of quercetin results in loss of functionality, poor activity, and reduced shelf-life of the bioactive component. In this regard, to improve the uptake, potential, and efficiency of natural components with prolonged storage in the host’s body after administration, numerous polymer drug delivery systems have been created. In the current study, three-dimensional (3D) porous (porosity: 92%; pore size: 81 µm) bio-polymeric foaming gelatin–alginate (GA) beads were fabricated for the entrapment of quercetin as therapeutic drug molecules—gelatin–alginate–quercetin (GAQ). The GAQ beads showed a significant uptake of quercetin molecules resulting in a reduction of reduced porosity up to 64% and pore size 63 µm with a controlled release profile in the PBS medium, showing ~80% release within 24 h. Subsequently, the GAQ beads showed remarkable antioxidant effects, and 95% anti-inflammatory activities along with remarkable in vitro cell culture growth and the observed proliferation of seeded fibroblast cells. Thus, we can conclude that the consistent release of quercetin showed non-toxic effects on normal cell lines and the bioactive surface of the GAQ beads enhances cell adhesion, proliferation, and differentiation more effectively than control GA polymeric beads and tissue culture plates (TCP). In summary, these findings show that these GAQ beads act as a biocompatible 3D construct with enormous potential in medicinal administration and tissue regeneration for accelerated healing.
Yazarlar (9)
1
Archna Dhasmana
ORCID: 0000-0002-1120-2009
2
Sumira Malik
ORCID: 0000-0001-5077-1493
3
Anuj Ranjan
ORCID: 0000-0003-2592-9716
4
Abhishek Chauhan
ORCID: 0000-0001-6475-1266
5
Hanaa M. Tashkandi
6
Shafiul Haque
ORCID: 0000-0002-2989-121X
7
Rajaa Al-Raddadi
ORCID: 0000-0002-8921-9628
8
Steve Harakeh
9
Gokhan Zengin
ORCID: 0000-0001-6548-7823
Anahtar Kelimeler
biocompatibility
drug
graft
herbal
quercetin
regeneration
Kurumlar
Amity University
Noida India
Amity University Jharkhand
Ranchi India
Faculty of Medicine
Jeddah Saudi Arabia
Jazan University
Jizan Saudi Arabia
Selçuk Üniversitesi
Selçuklu Turkey
Southern Federal University
Rostov-on-Don Russian Federation
Swami Rama Himalayan University
Dehradun India
Metrikler
5
Atıf
9
Yazar
6
Anahtar Kelime