Scopus
🔓 Açık Erişim
Antioxidant and Hepatoprotective Potential of Echinops ritro L. Extracts on Induced Oxidative Stress In Vitro/In Vivo
International Journal of Molecular Sciences · Haziran 2023
Makale Bilgileri
DergiInternational Journal of Molecular Sciences
Yayın TarihiHaziran 2023
Cilt / Sayfa24
Scopus ID2-s2.0-85163936110
Erişim🔓 Açık Erişim
Özet
Echinops ritro L. (Asteraceae) is traditionally used in the treatment of bacterial/fungal infections and respiratory and heart ailments. The aim of this study was to evaluate the potential of extracts from E. ritro leaves (ERLE) and flowering heads (ERFE) as antioxidant and hepatoprotective agents on diclofenac-induced lipid peroxidation and oxidative stress under in vitro and in vivo conditions. In isolated rat microsomes and hepatocytes, the extracts significantly alleviated oxidative stress by increasing cell viability and GSH levels and reducing LDH efflux and MDA production. During in vivo experiments, the administration of the ERFE alone or in combination with diclofenac resulted in a significant increase in cellular antioxidant protection and a decrease in lipid peroxidation witnessed by key markers and enzymes. A beneficial influence on the activity of the drug-metabolizing enzymes ethylmorphine-N-demetylase and aniline hydroxylase in liver tissue was found. In the acute toxicity test evaluation, the ERFE showed no toxicity. In the ultrahigh-performance liquid chromatography–high-resolution mass spectrometry analysis, 95 secondary metabolites were reported for the first time, including acylquinic acids, flavonoids, and coumarins. Protocatechuic acid O-hexoside, quinic, chlorogenic and 3, 5-dicaffeoylquinic acid, apigenin; apigenin 7-O-glucoside, hyperoside, jaceosidene, and cirsiliol dominated the profiles. The results suggest that both extracts should be designed for functional applications with antioxidant and hepatoprotective capacity.
Yazarlar (8)
1
Dimitrina Zheleva-Dimitrova
ORCID: 0000-0002-1952-9903
2
Rumyana Simeonova
ORCID: 0000-0003-4860-9053
3
Magdalena Kondeva-Burdina
4
Yonko Savov
5
Vessela Balabanova
ORCID: 0000-0002-9938-6542
6
Gokhan Zengin
ORCID: 0000-0001-6548-7823
7
Alexandra Petrova
8
Reneta Gevrenova
ORCID: 0000-0002-1254-2419
Anahtar Kelimeler
Echinops ritro
hepatoprotective potential
oxidative stress
secondary metabolites
UHPLC-HRMS
Kurumlar
Institute of Emergency Medicine “N. I Pirogov”
Sofia Bulgaria
Medical University of Sofia
Sofia Bulgaria
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
8
Atıf
8
Yazar
5
Anahtar Kelime