Scopus
🔓 Açık Erişim
Unraveling the chemical profile, antioxidant, enzyme inhibitory, cytotoxic potential of different extracts from Astragalus caraganae
Archiv der Pharmazie · Eylül 2023
Makale Bilgileri
DergiArchiv der Pharmazie
Yayın TarihiEylül 2023
Cilt / Sayfa356
Scopus ID2-s2.0-85164809970
Erişim🔓 Açık Erişim
Özet
Six extracts (water, ethanol, ethanol-water, ethyl acetate, dichloromethane, and n-hexane) of Astragalus caraganae were studied for their biological activities and bioactive contents. Based on high-performance liquid chromatography-mass spectrometry (HPLC-MS), the ethanol-water extract yielded the highest total bioactive content (4242.90 µg g−1), followed by the ethanol and water extracts (3721.24 and 3661.37 µg g−1, respectively), while the least total bioactive content was yielded by the hexane extract, followed by the dichloromethane and ethyl acetate extracts (47.44, 274.68, and 688.89 µg g−1, respectively). Rutin, p-coumaric, chlorogenic, isoquercitrin, and delphindin-3,5-diglucoside were among the major components. Unlike the dichloromethane extracts, all the other extracts showed radical scavenging ability in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay (8.73–52.11 mg Trolox equivalent [TE]/g), while all extracts displayed scavenging property in the 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging assay (16.18–282.74 mg TE/g). The extracts showed antiacetylcholinesterase (1.27–2.73 mg galantamine equivalent [GALAE]/g), antibutyrylcholinesterase (0.20–5.57 mg GALAE/g) and antityrosinase (9.37–63.56 mg kojic acid equivalent [KAE]/g) effects. The molecular mechanism of the H2O2-induced oxidative stress pathway was aimed to be elucidated by applying ethanol, ethanol/water and water extracts at 200 µg/mL concentration to human dermal cells (HDFs). A. caraganae in HDF cells had neither a cytotoxic nor genotoxic effect but could have a cytostatic effect in increasing concentrations. The findings have allowed a better insight into the pharmacological potential of the plant, with respect to their chemical entities and bioactive contents, as well as extraction solvents and their polarity.
Yazarlar (11)
1
Inci Kurt-Celep
2
Gokhan Zengin
ORCID: 0000-0001-6548-7823
3
Abdullahi Ibrahim Uba
4
Giovanni Caprioli
ORCID: 0000-0002-5530-877X
5
Ahmed M. Mustafa
6
Simone Angeloni
7
Ugur Cakilcioglu
8
Osman Guler
ORCID: 0000-0001-8225-1505
9
Alevcan Kaplan
10
Sharmeen Jugreet
11
Mohamad Fawzi Mahomoodally
Anahtar Kelimeler
antioxidant
Astragalus caraganae
bioactive compounds
extraction
solvents
Kurumlar
Acıbadem Mehmet Ali Aydınlar Üniversitesi
Istanbul Turkey
Batman University
Batman Turkey
İstanbul Arel Üniversitesi
Istanbul Turkey
Munzur University
Pertek Turkey
North-West University
Potchefstroom South Africa
Saveetha Dental College And Hospitals
Chennai India
Selçuk Üniversitesi
Selçuklu Turkey
Università degli Studi di Camerino
Camerino Italy
University of Mauritius
Reduit Mauritius
Metrikler
7
Atıf
11
Yazar
5
Anahtar Kelime