Scopus
🔓 Açık Erişim
The Phytochemical Profiling, In Vitro Antioxidant, and Hepatoprotective Activity of Prenanthes purpurea L. and Caffeoylquinic Acids in Diclofenac-Induced Hepatotoxicity on HEP-G2 Cells
International Journal of Molecular Sciences · Eylül 2023
Makale Bilgileri
DergiInternational Journal of Molecular Sciences
Yayın TarihiEylül 2023
Cilt / Sayfa24
Scopus ID2-s2.0-85172806396
Erişim🔓 Açık Erişim
Özet
Oxidative stress is a common phenomenon of many liver disorders; it both affects patient survival and directly influences the applicability, effectiveness, and toxicity of drugs. In the pursuit of reliable natural remedies for hepatoprotection, this study reports on the complete phytochemical characterization, antioxidant, and hepatoprotective activities of the Prenanthes purpurea methanol-aqueous extract in an in vitro model of diclofenac-induced liver injury (DILI). An ultra high-performance liquid chromatography–high-resolution mass spectrometry analysis (UHPLC-HRMS) was conducted, delineating more than 100 secondary metabolites for the first time in the species, including a series of phenolic acid-hexosides, acylquinic, acylhydroxyquinic and acyltartaric acids, and flavonoids. Quinic acid, chlorogenic, 3,5-dicaffeoylquinic and 5-feruloylhydroxyquinic acid, caffeoyltartaric and cichoric acids, eryodictiol-O-hexuronide, and luteolin O-hexuronide dominated the phytochemical profile and most likely contributed to the observed hepatoprotective activity of the studied P. purpurea leaf extract. The potency and molecular basis of cellular protection were investigated in parallel with pure caffeoylquinic acids in a series of pretreatment experiments that verified the antiapoptotic and antioxidant properties of the natural products.
Yazarlar (8)
1
Rositsa Mihaylova
ORCID: 0000-0001-5763-0730
2
Reneta Gevrenova
ORCID: 0000-0002-1254-2419
3
Alexandra Stefanova
4
Dimitrina Zheleva-Dimitrova
ORCID: 0000-0002-1952-9903
5
Vessela Balabanova
ORCID: 0000-0002-9938-6542
6
Gokhan Zengin
ORCID: 0000-0001-6548-7823
7
Rumyana Simeonova
ORCID: 0000-0003-4860-9053
8
Georgi Momekov
ORCID: 0000-0003-2841-7089
Anahtar Kelimeler
3,5-DiCQA
antioxidants
chlorogenic acid
hepatoprotection
hepatotoxicity
NSAIDs
polyphenols
Prenanthes parpurea
Kurumlar
Medical University of Sofia
Sofia Bulgaria
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
2
Atıf
8
Yazar
8
Anahtar Kelime