Scopus
Sweroside: An iridoid glycoside of potential neuroprotective, antidiabetic, and antioxidant activities supported by molecular docking
Amino Acids · Aralık 2023
Makale Bilgileri
DergiAmino Acids
Yayın TarihiAralık 2023
Cilt / Sayfa55 · 1765-1774
Scopus ID2-s2.0-85150458268
Özet
Oxidative stress can be a series burden on human health and may lead to many chronic diseases such as diabetes and neurological disorders. The use of natural products to scavenge the reactive oxygen species has attracted the attention of many researchers, to safely manage these conditions with fewer side effects, in available and cost-effective ways. The current study aimed at the isolation and structure elucidation of sweroside from Schenkia spicata (Gentianaceae) and the evaluation of its antioxidant, antidiabetic, neuroprotective, and enzyme inhibitory potential via in vitro and in silico studies. The antioxidant potential was evaluated by a variety of assays as ABTS, CUPRAC and FRAP, showing values of 0.34 ± 0.08, 21.14 ± 0.43, and 12.32 ± 0.20 mg TE/g, respectively, while demonstrating 0.75 ± 0.03 mmol TE/g for phosphomolybdenum (PBD) assay. Acetylcholinestrase (AChE), butyrylcholinesterase (BChE) and tyrosinase inhibitory activities were used to evaluate the neuroprotective effect, while the antidiabetic potential was evaluated by measuring α-amylase and glucosidase inhibitory activities. Results revealed that sweroside showed antioxidant and inhibitory effects on the enzymes tested with the exception of AChE. It demonstrated good tyrosinase inhibitory ability with 55.06 ± 1.85 mg Kojic acid equivalent /g. Regarding the antidiabetic ability, the compound displayed both amylase and glucosidase (0.10 ± 0.01 and 1.54 ± 0.01 mmol Acarbose equivalent/g, respectively) inhibitory activities. Molecular docking studies of sweroside on the active sites of the aforementioned enzymes in addition to NADPH oxidase were performed using Discovery Studio 4.1 software. Results revealed good binding affinities of sweroside to these enzymes mainly through hydrogen bonds and van der Waals interactions. Sweroside can be an important antioxidant and enzyme inhibitory supplement, yet further in vivo and clinical studies are required.
Yazarlar (8)
1
Gokhan Zengin
ORCID: 0000-0001-6548-7823
2
Mohamed El-Raey
3
Walaa El-Kashak
4
Gaber El Saber Batiha
5
Duaa Althumairy
6
Sarah Alamer
7
Nada M. Mostafa
8
Omayma A. Eldahshan
ORCID: 0000-0002-0972-0560
Anahtar Kelimeler
Antidiabetic
Antioxidant
Centaurium spicatum
Enzyme inhibition
Neuroprotective
Schenkia spicata
Sweroside
Kurumlar
Ain Shams University
Cairo Egypt
Faculty of Pharmacy - Ain Shams University
Cairo Egypt
Faculty of Veterinary Medicine
Damanhour Egypt
King Faisal University
Al-Ahsa Saudi Arabia
National Research Centre
Giza Egypt
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
10
Atıf
8
Yazar
7
Anahtar Kelime