Scopus
🔓 Açık Erişim
LC-MS/MS and GC-MS profiling, antioxidant, enzyme inhibition, and antiproliferative activities of Thymus leucostomus H ausskn. & V elen. extracts
Archiv der Pharmazie · Aralık 2023
Makale Bilgileri
DergiArchiv der Pharmazie
Yayın TarihiAralık 2023
Cilt / Sayfa356
Scopus ID2-s2.0-85172774319
Erişim🔓 Açık Erişim
Özet
The chemical composition as well as antioxidant, antiproliferative, and enzyme inhibition activities of extracts from aerial parts of Thymus leucostomus H ausskn. & V elen. obtained with hexane, methanol, and water were evaluated. Results showed that the methanol extract had significantly (p < 0.05) the highest total phenolic content (TPC; 107.80 mg GAE/g) and total flavonoids content (TFC; 25.21 mg RE/g) followed by the aqueous extract (102.72 mg GAE/g and 20.88 mg RE/g, respectively). LC-MS/MS-guided profiling of the three extracts revealed that rosmarinic acid (34.8%), hesperetin (42.9%), and linoleic acid (18%) were the dominant compounds in the methanol, aqueous and hexane extracts, respectively. GC-MS analysis of the hexane extract showed that ɣ-sitosterol (29.9%) was the major constituent. The methanol extract displayed significantly (p < 0.05) the highest Cu++, Fe+++, and Mo(VI) ions scavenging and reducing properties while the aqueous extract exerted significantly (p < 0.05) the highest metal chelating power (42.51 mg EDTAE/g). Both the hexane and methanol extracts effectively inhibited the acetylcholinesterase enzyme (2.63 and 2.65 mg GALAE/g, respectively) while the former extract exerted significantly (p < 0.05) the highest butyrylcholinesterase (2.32 mg GALAE/g), tyrosinase (19.73 mg KAE/g), and amylase (1.16 mmol ACAE/g) inhibition capacity. The aqueous extract exhibited the best glucosidase inhibition property (0.49 mmol ACAE/g). The methanol and hexane extracts exerted a higher cytotoxic effect on HT-29 (IC50: 8.12 µg/mL) and HeLa (IC50 = 8.08 µg/mL) cells, respectively. In conclusion, these results provide valuable insight into the potential use of T. leucostomus bioactive extracts in different pharmaceutical applications.
Yazarlar (11)
1
Shaimaa Fayez
2
Nouran M. Fahmy
ORCID: 0000-0003-0052-5096
3
Gokhan Zengin
ORCID: 0000-0001-6548-7823
4
Sakina Yagi
5
Abdullahi Ibrahim Uba
6
Omayma A. Eldahshan
ORCID: 0000-0002-0972-0560
7
Ismail Koyuncu
8
Ebru Temiz
9
Stefano Dall'Acqua
10
Stefania Sut
11
Selami Selvi
Anahtar Kelimeler
antioxidant
antiproliferative
chemical profile
enzyme inhibition
Thymus leucostomus
Kurumlar
Balikesir Üniversitesi
Balikesir Turkey
Faculty of Pharmacy - Ain Shams University
Cairo Egypt
Harran Üniversitesi
Sanliurfa Turkey
Harran University, Faculty of Medicine
Sanliurfa Turkey
İstanbul Arel Üniversitesi
Istanbul Turkey
Khartoum University
Khartoum Sudan
Selçuk Üniversitesi
Selçuklu Turkey
Università degli Studi di Padova
Padua Italy
Metrikler
2
Atıf
11
Yazar
5
Anahtar Kelime