Scopus
🔓 Açık Erişim
Characterising the Metabolomic Diversity and Biological Potentials of Extracts from Different Parts of Two Cistus Species Using UHPLC-MS/MS and In Vitro Techniques
Pathogens · Eylül 2024
Makale Bilgileri
DergiPathogens
Yayın TarihiEylül 2024
Cilt / Sayfa13
Scopus ID2-s2.0-85205080657
Erişim🔓 Açık Erişim
Özet
This study investigates the biochemical composition and biological properties of different parts (leaves, roots, and twigs) of two Cistus species (Cistus monspeliasis and Cistus parviflorus). The extracts were analysed using UHPLC-MS/MS to determine their chemical profiling. A range of antioxidant assays were performed to evaluate the extract’s antioxidant capabilities. The enzyme inhibition studies focused on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-amylase, and α-glucosidase and tyrosinase. In addition, the study examined the antimicrobial effects on different bacteria and yeasts and evaluated the toxicity using the MTT assay. Quinic acid, citric acid, gallic acid, catechin, quercetin derivatives, kaempferol, myricetin, ellagic acid, prodelphinidins, procyanidins, scopoletin, and flavogallonic acid dilactone are the main bioactive compounds found in both species. In enzyme inhibition assays, C. monspeliasis roots exhibited significant activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), with the values of 2.58 ± 0.02 mg GALAE/g and 11.37 ± 1.93 mg GALAE/g, respectively. Cytotoxicity studies showed mostly weak toxicity, with some samples moderately reducing viability in RAW and HepG2 cells. These findings underscore the diverse biochemical profiles and bioactive potential of Cistus species, suggesting their utility as natural sources of antioxidants and enzyme inhibitors for pharmaceutical and nutraceutical development.
Yazarlar (12)
1
Shakel Ahmed
2
Gokhan Zengin
ORCID: 0000-0001-6548-7823
3
Selami Selvi
4
Gunes Ak
5
Zoltán Cziáky
6
József Jekő
7
Maria João Rodrigues
8
Luisa Custodio
9
Roberto Venanzoni
ORCID: 0000-0002-7768-0468
10
Giancarlo Angeles Flores
ORCID: 0000-0001-6651-9794
11
Gaia Cusumano
12
Paola Angelini
ORCID: 0000-0002-6862-1079
Anahtar Kelimeler
antioxidants
chemical profiling
C. monspeliasis
C. parviflorus
enzyme inhibition
Kurumlar
Balikesir Üniversitesi
Balikesir Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Universidade do Algarve
Faro Portugal
Università degli Studi di Perugia
Perugia Italy
University of Nyíregyháza
Nyíregyháza Hungary