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Metabolomics-guided discrimination of constituents in five commercial apple (Malus × domestica Borkh.) cultivars: Assessment of their antioxidant, neuroprotective, and antidiabetic activities for potential use as functional food

Food Bioscience · Ekim 2024

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YÖKSİS Kayıtları
Metabolomics-guided discrimination of constituents in five commercial apple (Malus × domestica Borkh.) cultivars: Assessment of their antioxidant, neuroprotective, and antidiabetic activities for potential use as functional food
Food Bioscience · 2024 SCI-Expanded
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Makale Bilgileri

DergiFood Bioscience
Yayın TarihiEkim 2024
Cilt / Sayfa61
Özet Apples (Malus × domestica Borkh.) are available in different cultivars, we investigated the similarities and differences in the chemical composition and biological activities of the extracted apple oils by hydrodistillation and hexane extraction. The aroma constituents obtained by hydrodistillation and n-hexane extraction of five commercial Egyptian apple cultivars namely Pink Lady (Cripps pink), Red Delicious, Ambrosia, Golden Delicious, and Granny Smith were investigated by Gas chromatography-mass spectrometry (GC-MS). The total phenolic (TPC) and total flavonoid contents (TFC), in vitro antioxidant analysis, and enzyme inhibition assays were performed. Results showed that TPC and TFC were the highest in the hexane extract of pink ladies with values of 12.42 (mg GAE/g FW) and 1.35 (mg RE/g FW), respectively. Similarly, pink lady hexane extract showed the highest efficacy in antioxidant assays like 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), Cupric reducing antioxidant capacity (CUPRAC), ferric reducing antioxidant power (FRAP), metal chelation, and phosphomolybdenum tests with values of 25.21 mg TE/g, 39.82 mg TE/g, 19.27 mg TE/g, 28.16 mg EDTAE/g, and 1.36 mmol TE/g, respectively. Assessments of enzyme inhibition potential of the hexane extracts of the five cultivars on acetylcholinesterase (AChE) showed efficacy for ambrosia only with inhibition value at 2.53 mg GALAE/g. All other cultivars showed comparable efficacies in inhibiting tyrosinase, amylase, and glucosidase enzymes. Significant differences were observed in the chemical composition of the five apple cultivars which were reflected by differences in their antioxidant and enzyme inhibition capacities. Results of biological studies were further confirmed with in silico investigations.

Yazarlar (5)

1
Shaimaa Fayez
2
Omayma A. Eldahshan
ORCID: 0000-0002-0972-0560
3
Nilufar Nilofar
4
Gokhan Zengin
ORCID: 0000-0001-6548-7823
5
Nouran M. Fahmy
ORCID: 0000-0003-0052-5096

Anahtar Kelimeler

Antidiabetic Antioxidant Apple cultivars In silico studies Malus × domestica Borkh. Metabolomics Neuroprotective

Kurumlar

Ain Shams University
Cairo Egypt
Faculty of Pharmacy - Ain Shams University
Cairo Egypt
Selçuk Üniversitesi
Selçuklu Turkey
University of G. d'Annunzio Chieti and Pescara
Chieti Italy