Scopus
Cytotoxic, antioxidant, and enzyme inhibitory activities of Centaurea stapfiana extracts and their HPLC-ESI-QTOF-MS profiles: Insights into an unexplored Centaurea species
Fitoterapia · Aralık 2024
Makale Bilgileri
DergiFitoterapia
Yayın TarihiAralık 2024
Cilt / Sayfa179
Scopus ID2-s2.0-85203404702
Özet
The members of the genus Centaurea have a great interest in pharmaceutical and nutraceutical fields due to their biological potential. Based on this information, we aimed to evaluate the biological properties (antioxidant, enzyme inhibition and cytotoxicity) and chemical profile of the extract of Centaurea stapfiana, an unstudied species. The highest total phenolic content was found in the ethanol/water extract with 32.17 mg GAE/g. A total of 102 of them were identified by HPLC-ESI-QTOF-MS analysis. These compounds were mainly hydroxybenzoic acid and hydroxycinnamic acid as well as flavonoids. In the antioxidant tests, the ethanol/water extract had the best free radical scavenging and reducing ability. However, in the enzyme inhibition test, the ethanol extract was the most active. The extracts were also tested on two tumour cell lines (RAW 264.7 and HepG2) and one non-tumour cell line (S17). The ethanol extract showed the promising effect on HepG2 (cell viability: 28.6 % at 50 g/ml). Furthermore, we examined the interactions between the compounds and enzymatic and cellular targets. A good interaction was found between quercetin-3-xylosyl-(1- > 6)-glucoside and iNOS. In summary, our results suggest that C. stapfiana can be considered as a versatile raw material for the development of health-promoting applications in the pharmaceutical and cosmeceutical fields.
Yazarlar (12)
1
Gokhan Zengin
ORCID: 0000-0001-6548-7823
2
Álvaro Fernández-Ochoa
3
María de la Luz Cádiz-Gurrea
4
Francisco Javier Leyva Jiménez
5
Abdullahi Ibrahim Uba
6
Gunes Ak
7
Sanam Iram Soomro
8
Maruf Balos
9
Ugur Cakilcioglu
10
Maria João Rodrigues
11
Catarina Pereira
ORCID: 0000-0002-1131-8773
12
Luisa Custodio
Anahtar Kelimeler
Antioxidants
Centaurea
Flavonoids
Health-promoting agents
HepG2
iNOS
Kurumlar
İstanbul Arel Üniversitesi
Istanbul Turkey
Munzur University
Pertek Turkey
Sanlıurfa Provincial Directorate of National Education
Sanliurfa Turkey
Selçuk Üniversitesi
Selçuklu Turkey
The Aga Khan University
Karachi Pakistan
Universidad de Granada
Granada Spain
Universidade do Algarve
Faro Portugal