Scopus
A broad-spectrum biological activities of Heracleum humile extracts: A first report of the antiviral, anti-cancer and chemical properties
Food Bioscience · Aralık 2024
Makale Bilgileri
DergiFood Bioscience
Yayın TarihiAralık 2024
Cilt / Sayfa62
Scopus ID2-s2.0-85205326098
Özet
The current work focused on researching the bioactive potential of ethyl acetate (EA), methanol (MeOH), and water extract of H. humile plant, specifically ethyl acetate, methanol extracts were acquired with maceration method, and water extract was obtained by the infusion method. The highest total phenolic ingredient was determined in the water extract, while the highest total flavonoid ingredient was found in the MeOH extract. The water extract displayed the most potent antioxidant activity in the assays used. The ethyl acetate extract showed the highest enzyme inhibition activity in Butyrylcholinesterase inhibition (BChE) and amylase assay. Antibacterial efficiency was analyzed using microdilution analysis. The minimum inhibitory concentration (MIC) amounts ranged from 125 to 500 μg/mL except for water extract. The antiviral potential of the extracts was assessed against Bovine herpes virus 1 (BoHV-1) (Cooper strain). Methanol extract showed more potent antiviral effect. Selectivity indices were calculated for ethyl acetate and methanol extract as 3.32 and 13.39, respectively. All extracts displayed Deoxyribonucleic Acid (DNA) protection activity at approximately 25%–35%. For cell viability evaluation, 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) test was performed against triple-negative human breast adenocarcinoma cells (MDA-MB-231) and Michigan Cancer Foundation-7 (MCF-7) cells. The most effective results were found in ethyl acetate extract at 48h. The 50% inhibitor concentrations (IC50) were determined for MDA-MB-231 and MCF-7 as 116.7 μg/mL and 247.9 μg/mL, respectively. Based on the bioactive compound content results, extracts of H. humile include valuable compounds that possess significant biological properties. In conclusion, these extracts could offer promising novel plant-derived therapeutics.
Yazarlar (10)
1
Mustafa Ocal
2
Selda Duran Yelken
ORCID: 0000-0003-0633-3132
3
Yasemin Celik Altunoglu
4
Mehmet Cengiz Baloglu
5
Sanam Iram Soomro
6
Gokhan Zengin
ORCID: 0000-0001-6548-7823
7
Simone Angeloni
8
Ahmed M. Mustafa
9
Giovanni Caprioli
ORCID: 0000-0002-5530-877X
10
Mehmet Yavuz Paksoy
Anahtar Kelimeler
Anti-Cancer
Antioxidant
Antiviral
Heracleum humile
Maceration
Kurumlar
Faculty of Pharmacy
Zagazig Egypt
Kastamonu University
Kastamonu Turkey
Munzur University
Tunceli Turkey
Sabancı Üniversitesi
Tuzla Turkey
Selçuk Üniversitesi
Selçuklu Turkey
The Aga Khan University
Karachi Pakistan
Università degli Studi di Camerino
Camerino Italy