Scopus
🔓 Açık Erişim YÖKSİS DOI Eşleşti
SJR Q4
A cardioprotective role of Nerium oleander with the expression of hypoxia inducible factor 2A mRNA by increasing antioxidant enzymes in rat heart tissue
Acta Scientiae Veterinariae · Ocak 2018
YÖKSİS Kayıtları
A Cardioprotective Role of Nerium oleander with the Expression of Hypoxia Inducible Factor 2A mRNA by Increasing Antioxidant Enzymes in Rat Heart Tissue
Acta Scientiae Veterinariae · 2018 SCI-Expanded
Prof. Dr. BURAK DİK →
A Cardioprotective Role of Nerium oleander with the Expression of Hypoxia Inducible Factor 2A mRNA by Increasing Antioxidant Enzymes in Rat Heart Tissue
Acta Scientiae Veterinariae · 2018 SCI-Expanded
Prof. Dr. AYŞE ER →
A Cardioprotective Role of Nerium oleander with the Expression of Hypoxia Inducible Factor 2A mRNA by Increasing Antioxidant Enzymes in Rat Heart Tissue
Acta Scientiae Veterinariae · 2018 SCI-Expanded
Prof. Dr. AYŞE ER →
YÖKSİS Kayıtları — ISSN Eşleşmesi
Bovine Leukemia Virus Antibodies in Dairy Cattle Farms and Milk Cooling Tanks
2014 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. OĞUZHAN AVCI →
Effect of CorynebacteriumcutisLysate on Serum Oxidative Stressand Plasma Prostaglandin F2 Metabolite Levels
2014 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. BURAK DİK →
Hepatoprotective Effects of Ginseng in Rats Fed Cholesterol Rich Diet
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. ERCAN KESKİN →
Effect of Anti TNF on the Development of Offspringand Pregnancy Loss During Pregnancy in Rats
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. AYŞE ER →
Investigation of the Effect of Tarantula cubensis Extract on Acute Phase Response
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. AYŞE ER →
Detection of Antibodies against Equine Herpes Virus 1and Equine Herpes Virus 4 in Horses in Southeast Anatolia by Indirect Elisa
2014 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. OĞUZHAN AVCI →
Hepatoprotective Effects of Ginseng in Rats Fed Cholesterol Rich Diet
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. DENİZ ULUIŞIK →
Investigation of the Effect of Tarantula cubensis Extract on Acute Phase Response
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. BURAK DİK →
Effect of Anti TNF-a on the Development of Offspringand Pregnancy Loss During Pregnancy in Rats
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. BURAK DİK →
Amiodarone May Prevent the Tilmicosin caused Lethal Toxicity
2014 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. BÜNYAMİN TRAŞ →
Effect of Hoof Trimming on Milk Yield in Dairy Cows with Foot Disease
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. TAMER ÇAĞLAYAN →
Bovine coronavirus (BoCV) infection in calves with diarrhoea and their dams
2016 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. ORHAN YAPICI →
The Effects of Coenzyme Q10 on Inflammation Markers in Streptozotocin-Induced Diabetic Rats
2017 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. ERCAN KESKİN →
The Effects of Coenzyme Q10 on Inflammation Markersin Streptozotocin-Induced Diabetic Rats
2017 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. DENİZ ULUIŞIK →
Comparison of the Effectiveness of Halofuginone Lactate and Paromomycin in the Treatment of Calves Naturally Infected with Cryptosporidium parvum
2018 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. NERMİN IŞIK USLU →
Safety of antitheilerial drug buparvaquone in rams
2018 ISSN: 1678-0345 SCI-Expanded Q4
Prof. Dr. NERMİN IŞIK USLU →
Pentoxifylline May Restore Kanamycin-Induced Renal Damage in Rats
2018 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. ÖZGÜR ÖZDEMİR →
Comparison of the Effectiveness of Halofuginone Lactate and Paromomycin in the Treatment of Calves Naturally Infected with Cryptosporidium parvum
2018 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. ÖZLEM DERİNBAY EKİCİ →
Comparison of Thermographic Imaging and Other Diagnostic Techniques in Diagnosis of Cattle with Laminitis
2018 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. FATİH HATİPOĞLU →
Bovine Coronavirus (BoCV) Infection in Calves with Diarrhoea and Their Dams
2018 ISSN: 1678-0345 SCI-Expanded
Prof. Dr. OĞUZHAN AVCI →
Makale Bilgileri
ISSN16780345
Yayın TarihiOcak 2018
Cilt / Sayfa46
Scopus ID2-s2.0-85066410667
Erişim🔓 Açık Erişim
Özet
Background: Nerium oleander (NO) distillate is used to either protect heart cells against oxidative stress or reduce the risk of cardiovascular disease by regulating the production of reactive oxygen species. Hypoxia-inducible factors (HIFs) regulate cellular antioxidant defense mechanisms under hypoxic conditions in which heart cells survive; however, the key responsible mechanism of NO distillate for cardioprotection remains elusive. The objective of this study was to evaluate the effects on heart tissue at different time intervals after administering NO distillate intraperitoneally (IP) while considering the transcriptional regulation of HIFs and representative antioxidant enzymes. Materials, Methods & Results: The NO plant was chopped, and distillated water was added. The mixture was distilled, and the distillate separated and collected into tubes, after which it was lyophilized to obtain dry material. Twenty male Wistar albino rats (2-3 month-old, 250-300 g each) were used in the study. The rats were randomly divided into four groups. The control group (n = 5) received IP injections of saline; the remaining 15 rats received IP injections of a single dose of 7.5 mL NO distillate. The NO distillate injected rats were divided into three groups according to the time from injection to harvest the heart tissue samples. The tissues were collected at 0 h (control; n = 5), 2 h (group 2; n = 5), 4 h (group 3; n = 5), and 8 h (group 4; n = 5) after injection and under general anesthesia (60 mg/kg ketamine, IP + 10 mg/kg xylazine, IP). Quantitative polymerase chain reaction (qPCR) was used to assess the expression profiles of the genes of interest in the heart tissues. Hypoxanthine phosphoribosyltransferase was used as the reference gene. The expression of manganese superoxide dismutase (MnSOD) mRNA was in a steady state level between the control group and group 2 (P > 0.05); however, it significantly increased in group 3 and 4 compared with that in the control (P < 0.05). Expression of catalase (CAT) mRNA was significantly higher in group 2 than in the control group (P < 0.05) although it was lower in group 3 and 4 than in group 2 (P < 0.05); however, it appeared to be similar among the control group, group 3, and group 4 (P > 0.05). Copper (Cu) SOD mRNA was equally expressed in both the control group and group 2 (P > 0.05) but was lower in group 3 and 4 than in group 2 (P < 0.05). Expressions of HIF1A, HIF2A, and HIF3A mRNA were detected in the rat heart tissues in the control and 2, 4, and 8 h after administration of NO distillate. Expression of HIF1A mRNA was in a steady state and did not differ among groups 2, 3, and 4 (P > 0.05). Similarly, the expression of HIF2A mRNA did not change between the control group and group 2 (P > 0.05); however, it was higher in group 3 than in the control (P < 0.05) and tended to be higher in group 3 than in group 2 (P = 0.063). HIF3A mRNA expression did not change significantly in the heart tissue of any of the groups (P > 0.05). Discussion: The present study using rats determined that MnSOD, CAT, CuSOD, HIF1A, HIF2A, and HIF3A mRNA are expressed in the heart tissues after administration of NO distillate. The increased expression of HIF2A mRNA after 4 h in accordance with a rise in CAT mRNA after 2 h, and MnSOD mRNA after 4 and 8 h might confirm the role of HIF2A mRNA in oxidative stress defense by regulating antioxidant enzymes; consequently, this study may expand our understanding of uses of NO distillate with respect to molecular pathways.
Yazarlar (7)
1
Mustafa Hitit
2
Orhan Corum
ORCID: 0000-0003-3168-2510
3
D. Durna Corum
4
Huseyin Donmez
5
Gul Cetin
6
Burak Dik
7
A. Er
Anahtar Kelimeler
Antioxidant enzymes
Cardioprotection
Gene expression
Hypoxia inducible factors
Nerium oleander
Kurumlar
Erzincan Binali Yıldırım Üniversitesi
Erzincan Turkey
Kastamonu University
Kastamonu Turkey
Necmettin Erbakan Üniversitesi
Meram Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Scimago Dergi (ISSN Eşleşmesi)
Acta Scientiae Veterinariae
Q4
SJR Skoru0,155
H-Index20
YayıncıUniversidade Federal do Rio Grande do Sul
ÜlkeBrazil
Veterinary (miscellaneous) (Q4)
Metrikler
10
Atıf
7
Yazar
5
Anahtar Kelime