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Effect of doxycycline and meloxicam on cytokines, brain-derived neurotrophic factor, matrix metalloproteinase-3, tissue inhibitor of metalloproteinase-3 and cyclooxygenase-2 in brain

Iranian Journal of Basic Medical Sciences · Ekim 2020

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YÖKSİS Kayıtları
Effect of doxycycline and meloxicam on cytokines, brain-derived neurotrophic factor, matrixmetalloproteinase-3, tissue inhibitor of metalloproteinase-3 and cyclooxygenase 2 in brain.
Iranian Journal of Basic Medical Sciences · 2020 SCI-Expanded
PROFESÖR AYŞE ER →
Effect of doxycycline and meloxicam on cytokines, brain-derived neurotrophic factor, matrixmetalloproteinase-3, tissue inhibitor of metalloproteinase-3 and cyclooxygenase 2 in brain.
Iranian Journal of Basic Medical Sciences · 2020 SCI-Expanded
PROFESÖR BURAK DİK →
Effect of doxycycline and meloxicam on cytokines, brain-derived neurotrophic factor, matrix metalloproteinase-3, tissue inhibitor of metalloproteinase-3 and cyclooxygenase-2 in brain
IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES · 2020 SCI-Expanded
PROFESÖR BURAK DİK →
sadasda
iranian · 2020 SCI-Expanded
PROFESÖR BURAK DİK →
Effect of doxycycline and meloxicam on cytokines, brain-derived neurotrophic factor, matrix metalloproteinase-3, tissue inhibitor of metalloproteinase-3 and cyclooxygenase-2 in brain
IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES · 2020 SCI-Expanded
PROFESÖR BURAK DİK →

Makale Bilgileri

DergiIranian Journal of Basic Medical Sciences
Yayın TarihiEkim 2020
Cilt / Sayfa23 · 1328-1334
Özet Objective(s): Prevention of inflammation in early stages will be useful in maintaining vitality of the organism. The objective of this study was to evaluate the effects of doxycycline (DOX) or meloxicam (MLX) monotherapy and combination therapy on the levels of inflammatory mediators in the brain tissues of rats with Escherichia coli lipopolysaccharide (LPS)-induced brain inflammation. Materials and Methods: Seventy-eight rats were divided into the following groups: control (n=6), LPS (0.5 µg/10 µl intracranial) (n=18), LPS (0.5 µg/10 µl intracranial)+DOX (40 mg/kg intraperitoneal) (n=18), LPS (0.5 µg/10 µl intracranial)+MLX (2 mg/kg intraperitoneal) (n=18) and LPS (0.5 µg/10 µl intracranial)+DOX (40 mg/kg intraperitoneal)+MLX (2 mg/kg intraperitoneal) (n=18) groups. Brain tissues were harvested from all rats in the control group and from six rats each in the four experimental groups at 1, 3 and 6 hr under anaesthesia. The levels of tumor necrosis factor α (TNFα), interleukin 4 (IL-4), IL-6, IL-10, IL-17, brain-derived neurotrophic factor (BDNF), matrix metalloproteinase 3 (MMP-3), tissue inhibitor of metalloproteinase 3 (TIMP-3) and cyclooxygenase 2 (COX-2) in the brain tissues were measured using ELISA kits with ELISA device. Results: LPS administration increased proinflammatory cytokines (TNF, IL-6, IL-17), and MMP-3 levels and decreased anti-inflammatory cytokines (IL-10, IL-4), and BDNF levels. The lowest TNFα levels were detected in the LPS+MLX group (P<0.05). All the drug treatment groups showed decreased IL-17 and COX-2 levels compared to the LPS groups. Conclusion: DOX or MLX monotherapy exerts neuroprotective effects against brain inflammation by decreasing proinflammatory cytokine levels and by increasing anti-inflammatory cytokines levels.

Yazarlar (4)

1
A. Er
2
Devran Coskun
ORCID: 0000-0003-1151-1861
3
Emre Bahcivan
4
Burak Dik

Anahtar Kelimeler

Brain Doxycycline Inflammation Meloxicam Neuroprotective

Kurumlar

Kafkas Üniversitesi, Veteriner Fakültesi
Kars Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Siirt Üniversitesi
Siirt Turkey

Metrikler

6
Atıf
4
Yazar
5
Anahtar Kelime

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