Scopus
YÖKSİS Eşleşti
Raffinose and hypotaurine improve the post-thawed Merino ram sperm parameters
Cryobiology · Ağustos 2013
YÖKSİS Kayıtları
Raffinose and hypotaurine improve the post thawed Merino ram sperm parameters
Cryobiology · 2013 SCI-Expanded
PROFESÖR AHMED NURİ KURŞUNLU →
Makale Bilgileri
DergiCryobiology
Yayın TarihiAğustos 2013
Cilt / Sayfa67 · 34-39
Scopus ID2-s2.0-84879553329
Özet
The aim of this study was to determine the effects of raffinose and hypotaurine on sperm parameters after the freeze-thawing of Merino ram sperm. Totally 40 ejaculates of five Merino ram were used in the study. Semen samples, which were diluted with a Tris-based extender containing 10. mM raffinose, 5. mM hypotaurine, 5. mM raffinose +2.5. mM hypotaurine (H + R) and no antioxidant (control), were cooled to 5 °C and frozen in 0.25. ml French straws and stored in liquid nitrogen. Frozen straws were then thawed individually at 37 °C for 25. s in a water bath for evaluation. The addition of raffinose led to higher percentages of subjective and CASA motilities (47.5 ± 12.2%, 46.3 ± 13.6%) compared to controls (38.8 ± 13.8%, 30.5 ± 11.7%, P< 0.05). For the CASA progressive motility, 5. mM raffinose (20.12 ± 8.82%) had increasing effect in comparison to control (10 ± 7.94%, P< 0.05) following the freeze-thawing process. Raffinose and hypotaurine led to higher viability (40.8 ± 4.68%, 40.8 ± 4.7%), high sperm mitochondrial activity (29.5 ± 5.4%, 27.3 ± 4.9%) and acrosome integrity (50.8 ± 8.1, 50.7 ± 4.4) percentages, compared to control groups (31.5 ± 3.5%, 9.5 ± 8.2%, 42.8 ± 7.3%, P< 0.05). H + R group only led to high sperm mitochondrial activity when compared to control group. In the comet test, raffinose and hypotaurine resulted in lower sperm with damaged DNA (6.2% and 3.9%) than that of control (9.1%), reducing the DNA damage. For TUNEL assay, The TUNEL-positive cell was distinguished by distinct nuclear staining. Raffinose and H + R groups resulted in lower sperm with TUNEL-positive cell (1.5 ± 1.2% and 2.1 ± 0.9%) than that of control (4.9 ± 2.5%) (P< 0.05). In conclusion, findings of this study showed that raffinose and hypotaurine supplementation in semen extenders provided a better protection of sperm parameters against cryopreservation injury, in comparison to the control groups. © 2013.
Yazarlar (9)
1
Mustafa Numan Bucak
2
Nazan Keskin
3
M. Taşpinar
4
Kenan Çoyan
5
N. Baspinar
6
Mihai Cenariu
7
A. Bilgili
8
Caner Ozturk
9
Ahmed Nuri Kursunlu
Anahtar Kelimeler
Additives
Comet test
Flourescent staining parameters
Motility
Ram sperm
TUNEL-positive cells
Kurumlar
Ankara Üniversitesi
Ankara Turkey
Pamukkale Üniversitesi
Denizli Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Universitatea de Medicina si Farmacie Iuliu Hatieganu din Cluj-Napoca
Cluj Napoca Romania
Van Yüzüncü Yıl Üniversitesi
Van Turkey
Metrikler
49
Atıf
9
Yazar
6
Anahtar Kelime