Scopus
YÖKSİS DOI Eşleşti
SJR Q1
Epitope mapping of Campylobacter jejuni flagellar capping protein (FliD) by chicken (Gallus gallus domesticus) sera
Comparative Immunology Microbiology and Infectious Diseases · Aralık 2016
YÖKSİS Kayıtları
Epitope mapping of Campylobacter jejuni flagellar capping protein (FliD) by chicken (Gallus gallus domesticus) sera
Comparative Immunology, Microbiology and Infectious Diseases · 2016 SCI-Expanded
Doç. Dr. ARİFE EZGİ TELLİ →
YÖKSİS Kayıtları — ISSN Eşleşmesi
Epitope mapping of Campylobacter jejuni flagellar capping protein (FliD) by chicken (Gallus gallus domesticus) sera
2016 ISSN: 0147-9571 SCI-Expanded Q3
Doç. Dr. ARİFE EZGİ TELLİ →
A one-year descriptive epidemiology of zoonotic abortifacient pathogen bacteria in farm animals in Turkey
2021 ISSN: 0147-9571 SCI-Expanded Q2
Doç. Dr. ASLI BALEVİ →
A one-year descriptive epidemiology of zoonotic abortifacient pathogen bacteria in farm animals in Turkey
2021 ISSN: 0147-9571 SCI-Expanded Q1
Doç. Dr. ALİ USLU →
A one-year descriptive epidemiology of zoonotic abortifacient pathogen bacteria in farm animals in Turkey
2021 ISSN: 0147-9571 SCI-Expanded Q2
Dr. Öğr. Üyesi YASEMİN KARYEYEN →
A one-year descriptive epidemiology of zoonotic abortifacient pathogen bacteria in farm animals in Turkey
2021 ISSN: 0147-9571 SCI-Expanded Q2
Prof. Dr. ZAFER SAYIN →
A one-year descriptive epidemiology of zoonotic abortifacient pathogen bacteria in farm animals in Turkey
2021 ISSN: 0147-9571 SCI-Expanded Q2
Prof. Dr. OSMAN ERGANİŞ →
Makale Bilgileri
ISSN01479571
Yayın TarihiAralık 2016
Cilt / Sayfa49 · 76-81
Scopus ID2-s2.0-84993968419
Özet
Campylobacter jejuni, a Gram-negative rod, is a zoonotic pathogen associated with human acute bacterial gastroenteritis worldwide. The flagellum, composed of more than 35 proteins, is responsible for colonization of C. jejuni in the host gastrointestinal tract as well as inducing protective antibodies against the homologous serotype. In our previous study, we demonstrated that the flagellar capping protein (FliD) is an immunodominant protein that reacted strongly to sera from field chickens. In this communication, we mapped linear immunoreactive epitopes on FliD using a set of 158 synthetic peptides of 15-mer overlapping with 11 amino acid residues on peptide microarrays with sera from field chickens. The results from peptide microarrays showed (1) no cross-reactivity of the immobilized peptides with the secondary anti-chicken antibody in the control incubation, and (2) heterogeneous patterns of sera reacting to the immobilized peptides. The peptides that reacted to more than three chicken sera and had higher averages of fluorescence units were selected for further validation by the peptide ELISA. The results showed peptides 24, 91 and 92 had relatively high reactivity and less variation among 64 individual serum samples, indicating these peptides represented the shared immunodominant epitopes on the C. jejuni FliD protein. These peptides were also recognized by sera from chickens immunized with the purified recombinant FliD protein. The findings of the specific shared linear immunodominant epitopes on FliD in this study provide a rationale for further evaluation to determine their utility as epitope vaccines covering multiple serotypes for chicken immunization, and subsequently, for providing safer poultry products for human consumption.
Yazarlar (6)
1
Hung Yueh Yeh
ORCID: 0000-0003-0806-2428
2
A. Ezgi Telli
3
Jarra F. Jagne
4
Christopher L. Benson
5
Kelli L. Hiett
6
John E. Line
Anahtar Kelimeler
Campylobacter jejuni
Epitope mapping
Flagellar capping protein
FliD
Foodborne pathogen
Zoonosis
Kurumlar
Cornell University
Ithaca United States
Selçuk Üniversitesi
Selçuklu Turkey
University of Georgia
Athens United States
USDA Agricultural Research Service
Washington, D.C. United States
Scimago Dergi (ISSN Eşleşmesi)
Comparative Immunology, Microbiology and Infectious Diseases
Q1
SJR Skoru0,596
H-Index72
YayıncıElsevier Ltd
ÜlkeUnited Kingdom
Veterinary (miscellaneous) (Q1)
Infectious Diseases (Q2)
Medicine (miscellaneous) (Q2)
Immunology (Q3)
Immunology and Allergy (Q3)
Microbiology (Q3)
Metrikler
5
Atıf
6
Yazar
6
Anahtar Kelime