Scopus
YÖKSİS Eşleşti
Design of an electrochemical biosensing system for xanthine detection and a study on binding interaction of ketoconazole with xanthine oxidase
Colloids and Surfaces A: Physicochemical and Engineering Aspects · Mart 2014
YÖKSİS Kayıtları
Design of an electrochemical biosensing system for xanthine detection and a study on binding interaction of ketoconazole with xanthine oxidase
Colloids and Surfaces A: Physicochemical and Engineering Aspects · 2014 SCI-Expanded 1 atıf
PROFESÖR SALİH ZEKİ BAŞ →
Design of an electrochemical biosensing system for xanthine detection and a study on binding interaction of ketoconazole with xanthine oxidase
Colloids and Surfaces A: Physicochemical and Engineering Aspects · 2014 SCI-Expanded
PROFESÖR ESRA MALTAŞ ÇAĞIL →
Makale Bilgileri
DergiColloids and Surfaces A: Physicochemical and Engineering Aspects
Yayın TarihiMart 2014
Cilt / Sayfa444 · 40-47
Scopus ID2-s2.0-84891951115
Özet
Xanthine oxidase (XO) was successfully immobilized by covalent attachment on poly(glycidyl methacrylate-co-vinylferrocene) (P(GMA-co-VFc)), a redox copolymer containing pendant epoxy and ferrocene moieties, for the evaluation of both the biosensing properties and the effect of the interaction of ketoconazole (Ktc) with the immobilized XO. The binding interaction between Ktc, a drug used to treat fungal infections, and the immobilized XO on P(GMA-co-VFc) was also studied by fluorescence spectroscopy technique. The binding capacity of the drug was determined using a calibration curve equation that was drawn at excitation wavelength of 300nm using fluorescence spectroscopy. The interaction ability was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and native-PAGE analysis. The enzyme electrode exhibited a linear range from 2.7×10-3 to 0.55mM with a sensitivity of 19.42μAmM-1cm-2 and a detection limit of 8×10-4mM for the detection of xanthine. The activation energy (Ea) and the apparent Michaelis-Menten constant (Kmapp) values were found to be 12.30kJmol-1 and 0.38mM, respectively. © 2013 Elsevier B.V.
Yazarlar (4)
1
Salih Zeki Bas
ORCID: 0000-0003-2822-8851
2
Esra Maltas Cagil
3
Busra Sennik
4
Faruk Yilmaz
Anahtar Kelimeler
Biosensor
Fluorescence
Ketoconazole
Protein-drug interaction
Xanthine oxidase
Kurumlar
Gebze Teknik Üniversitesi
Gebze Turkey
Istanbul Medeniyet University
Istanbul Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
16
Atıf
4
Yazar
5
Anahtar Kelime