Scopus
YÖKSİS Eşleşti
Design of a probe based on poly(glycidyl methacrylate-co-vinylferrocene)- coated Pt electrode for electrochemical detection of PTEN gene in PCR amplified samples from prostate tissues
Journal of Applied Polymer Science · Ağustos 2014
YÖKSİS Kayıtları
title Design of a probe based on poly glycidyl methacrylate i co i vinylferrocene coated Pt electrode for electrochemical detection of PTEN gene in PCR amplified samples from prostate tissues title
Journal of Applied Polymer Science · 2014 SCI-Expanded
PROFESÖR ESRA MALTAŞ ÇAĞIL →
Design of a probe based on poly glycidyl methacrylate co vinylferrocene coated Pt electrode for electrochemical detection of PTEN gene in PCR amplified samples from prostate tissues
Journal of Applied Polymer Science · 2014 SCI-Expanded
PROFESÖR ESRA MALTAŞ ÇAĞIL →
Design of a probe based on poly glycidyl methacrylate co vinylferrocene coated Pt electrode for electrochemical detection of PTEN gene in PCR amplified samples from prostate tissues
Journal of Applied Polymer Science · 2014 SCI-Expanded
PROFESÖR SALİH ZEKİ BAŞ →
Makale Bilgileri
DergiJournal of Applied Polymer Science
Yayın TarihiAğustos 2014
Cilt / Sayfa131
Scopus ID2-s2.0-84901194183
Özet
In this study, a new probe based on immobilization of amino linked oligonucleotide (NH2-linked DNA) on poly(glycidyl methacrylate-co-vinylferrocene)-coated Pt electrode was fabricated for the electrochemical detection of PTEN gene from human prostate tissues. The experimental parameters such as DNA immobilization time, DNA concentration, and target concentration were optimized. The selectivity of the NH 2-linked DNA probe was assessed with mismatch (MM) and noncomplementary (NC) sequences. The applicability of the NH2-linked DNA probe to the PCR amplified samples correspond to PTEN gene from prostate tissues was evaluated. The immobilization of DNA on the copolymer was confirmed by FTIR, AFM, CV and DPV analysis. The PCR products were also identified by using agarose gel electrophoresis. The prepared probe indicated a linear range (10-100 μg mL-1) with a detection limit (4.7 μg mL -1) and a good selectivity of the NH2-linked DNA probe toward target DNA sequence. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40638. Copyright © 2014 Wiley Periodicals, Inc.
Yazarlar (5)
1
Salih Zeki Bas
ORCID: 0000-0003-2822-8851
2
Esra Maltas Cagil
3
Busra Sennik
4
Faruk Yilmaz
5
Hasibe Cingilli Vural
Anahtar Kelimeler
biomaterials
nucleic acids
sensors and actuators
Kurumlar
Gebze Teknik Üniversitesi
Gebze Turkey
Istanbul Medeniyet University
Istanbul Turkey
Selçuk Üniversitesi
Selçuklu Turkey