In this study, the authors aimed to determine the prenatal comfort and quality of life levels of pregnant women with and without headaches. We conducted a descriptive study and collected online data from 425 pregnant women via social media between April and October 2022. The Personal Information Form, Prenatal Comfort Scale, and Quality of Life Scale-WHOQOL-BREF (TR) were used as data collection tools. Among the participants, 59.5% reported experiencing headaches during pregnancy. The mean scores on the Prenatal Comfort Scale were 64.28 ± 11.37 for those with headaches and 65.87 ± 9.44 for those without, with no significant difference (p = 0.133). However, significant differences were observed in the physical (p < 0.001), psychological (p = 0.002), and environmental (p = 0.012) sub-dimensions of the WHOQOL-BREF (TR), where participants with headaches had lower scores. While headaches did not significantly affect prenatal comfort, these findings indicate that they negatively impacted quality of life in physical, psychological, and environmental domains.