Aim: Cardiac arrest (CA) is a leading cause of mortality worldwide, with survival outcomes heavily dependent on prompt recognition and intervention. Basic Life Support (BLS) training is critical for equipping healthcare professionals with the skills necessary to manage such emergencies effectively. Tailored BLS training is essential in dentistry, where emergencies like CA, though rare, can have severe consequences. This study evaluated the effectiveness of a structured BLS training program for dental residents at Selçuk University Faculty of Dentistry, incorporating theoretical, practical, and video-based instruction components. Methods: A total of 151 volunteer dental residents participated in a structured BLS training program conducted between May 14-17, 2024. Training included theoretical lectures, hands-on practice on mannequins, and a preparatory video covering BLS interventions for adults, children, and infants. Knowledge acquisition was measured using pre-tests, post-tests, and a retention test one month after training. Participants’ demographic data and prior BLS experience were also evaluated. Results: Participants demonstrated significant improvement in knowledge immediately after training, with retention remaining above pre-test levels one month later. In analyzing the number of participants answering the questions correctly in the post-training tests, p<0.001 was found for 10 out of 12 questions. Those watching the preparatory video scored significantly higher in post-training and retention tests than those who did not, highlighting the supportive role of the videos. Most participants (91.8%) reported never performing BLS in real-life scenarios, underscoring the need for recurring hands-on training to address gaps in practical readiness. Conclusion: This study shows that structured BLS training effectively improves knowledge acquisition and retention in dental residents. Preparatory videos aid learning. To sustain knowledge and readiness, periodic refreshers, advanced simulation technologies, and standardized BLS curricula are recommended. Incorporating BLS training into dental education helps professionals respond confidently in emergencies, enhancing patient’s safety outcomes.