Introduction: The study evaluated the effect of the vaccine education program developed using the Integrated Change Model on mothers' vaccine literacy and advocacy. Methods: The study is a parallel design randomized controlled trial with a waiting-list. Seventy-four mothers were enrolled in the study. In the study, the experimental group received a four-session vaccine education program, one week apart. The study used mixed ANOVA to explore the interaction between time and group. Effect sizes were interpreted using partial eta squared. Results: Vaccine literacy increased significantly in the intervention group in the post-test, and the intervention had a large effect size. In addition, it was found that vaccine knowledge increased with a large effect size in the intervention group, positive changes in vaccine attitudes, and vaccine advocacy intentions increased. Discussion: The Integrated Change Model vaccine education program can be used to reduce maternal and community vaccine hesitancy and increase vaccine acceptance. Reporting Method: The CONSORT-Outcomes 2022 Extension. Protocol Registration: ClinicalTrials NCT05929053.