The goal of this research was aimed to investigate the nutraceutical and bioactive compounds of Gundelia seed and its seed oils. The contents of fatty acids, triglycerides, sterols, tocopherol, tocotrienol (tocols) and some physicochemical properties of the cold pressed Gundelia seed oils were analyzed by HPLC and GC devices. The main fatty acid was found to be linoleic acid (45.078-75.113%), which is a polyunsaturated fatty acid (PUFA), and the main triglyceride structure was LLL (trilinolein, 13.39-44.6%). The main sterol was β-sterol (37.65-72.62%) and the main tocol was α-tocopherol (31.10-77.95 µg/g). It was determined that the total phenolic content of the oils of Gundelia seeds varied between 974.55 and 3439.09 µg GAE/mg oil and their antioxidant capacity ranged between 113.56 and 287.26 mg trolox/l. The oils obtained from the seeds of different Gundelia have similar properties to some edible oils and have a rich content in terms of nutraceutical and bioactive compounds.