The aim of this study was to determine the effects of alone and combined treatment of Tarantula cubensis alcoholic extract (TCAE) and Methenolone Enanthate (ME) on morphometric parameters of humerus and femur in young rats. In this study, 36 five–week–old Wistar Albino rats were used. The animals were divided into 4 groups; Control group (n:6, normal saline solution, 0.2 mL·rat-1, subcutaneously –SC–), TCAE group (Tarantula cubensis alcoholic extract, 0.2 mL·rat-1, SC), ME group (Methenolone Enanthate), 10 mg·kg-1, intramuscularly –IM–) and TCAE + ME group (Tarantula cubensis alcoholic extract, 0.2 mL·rat-1, SC + Methenolone Enanthate, 10 mg·kg-1, IM). Drug treatments were done once a week for 7 weeks. At the end of the experiment, all the animals were euthanized and their humerus and femur bones were resected and their morphometric parameters were determined. No statistical differences were determined (P>0.05) between the groups in terms of the anatomical reference points (length, corpus thickness, cavum medullare diameter, and cortex thickness) of humerus and femur bones. In conclusion, it was found that alone and combined treatments of Tarantula cubensis alcoholic extract and ME (at normal dose) had no effects on morphometric parameters of humerus and femur bones in rats in the growing period. However, these effects may not be observed at high–dose and long–term treatments in rats.