UiO-66-NH2 material is a variant of Zr-based MOF most widely used for various applications, exhibiting unprecedented excellent hydrothermal and physicochemical stability. In this study, after UiO-66-NH2 reacted with chlorosulfonyl isocyanate, the fluorescent UiO-66-NG probe was prepared by interacting with the N-methylglucamine molecule. The structure of the prepared probe was confirmed by characterizing them with techniques such as FTIR, SEM, and XRD. The sensing properties of this prepared probe against different anions and cations were investigated and it was understood that it showed sensitive selectivity only for H3BO3. The H3BO3 detection limit (LOD) of the UiO-66-NG probe was determined as 1.81 μM. Boric acid was determined in real samples by using tap water, lake water, and river water. Fluorescence imaging was performed using the plant Lepidium sativum for the detection of boric acid in aqueous medium and for bio-imaging in a living plant system. These results show that the prepared UiO-66-NG can be used successfully in the determination of H3BO3 in living plants.