Aim: To determine the knowledge and attitudes of adult intensive care nurses regarding pain. Method: This descriptive and cross-sectional study was conducted with 196 nurses working in the intensive care units of a tertiary university hospital between June 2022 and September 2022. Data were collected by face-to-face interview method, and the “Personal Information Form” and “Nurses’ Knowledge and Attitude Scale Regarding Pain” were used as data collection tools. Results: About 71.8% of the nurses were between the ages of 18 and 30, 58.5% were women, 54.9% had a bachelor's degree, and 55.1% had been working in intensive care for 0-5 years. The nurses' total knowledge and attitude score levels were 11.8% inadequate, 64.1% moderate, and 24.1% good. A statistically significant relationship was found between age, gender, receiving training on pain in the institution, satisfaction level with the unit in which one works, frequency of pain assessment and indicators taken into consideration when evaluating pain severity, and the total scale score average (p < 0.05). Conclusions: The average pain knowledge and attitude scores of intensive care nurses are at a good level. Results can be further improved with planned training on pain.