Squamous cell carcinoma (SCC) is the most common malignant tumor of the eyelid in cats. It is usually seen in white-haired cats aged 10 years and older. Although various regional and systemic chemotherapy treatments have been proposed, surgical total resection of the mass is generally recognized as the most accepted treatment method. The aim of this study was to evaluate the treatment of eyelid SCC by surgical total resection in a cat. The study material consisted of a short-haired female grey-white stray cat that was brought to Selçuk University, Faculty of Veterinary Medicine, Animal Hospital, Department of Surgery on January 4, 2024 with the complaint of ulcerative tumor in the right eye. After clinical and hematologic examinations, the cat was operated for mass resection. A dose of 0.25 ml bupivacaine was injected subconjuctivally into the bulbar conjunctiva of the T-shaped cartilage, and then the conjunctival tissue from which the third eyelid originated was dissected along all its borders with dissecting scissors and total resection of the tissue was achieved. The final diagnosis of SCC was made after histopathologic examination. Postoperative detailed examination of the eye segments and ocular MR imaging revealed no pathology. In addition, according to the patient's clinical and hematologic findings and radiographic findings of the thorax, no evidence of metastasis was found. During the 1-month postoperative follow-up period, no recurrence or general condition deterioration was detected. In conclusion, we believe that surgical total resection of the third eyelid is a feasible method for the treatment of SCC originating from the third eyelid in cats.