Objective: The aim of this study is the assessment of pregnant women’s anxiety and comfort level during the NST procedure. Methods: This study is a descriptive study. The study population consisted of all pregnant women who accepted to work at a University Hospital in Konya/Türkiye, affiliated to the Ministry of Health, between November 2021 and February 2022 and enrolled in the NST polyclinic. The study sample consisted of 331 volunteer pregnant women who were over 18 years of age and who presented to the NST Polyclinic after the 32nd gestational week. The data collection instruments used were the personal information form, the NST Evaluation Form, the State and Trait Anxiety Inventory, and the Prenatal Comfort Scale. Results: It was found that the occurrence of problems during pregnancy (nausea, vomiting, etc.) and gravida were variables that influenced the anxiety level of pregnant women. Pregnant women’s body mass index, the occurrence of problems during pregnancy, gravida, number of births, knowledge of the purpose of the NST procedure, and mother’s position of the during the NST procedure are the factors that influence pregnant women’s comfort level. Conclusion: Consistent with these results, it is important to determine the factors that influence the anxiety and comfort level of pregnant women.