In this study, we prepared a facile and selective Bluetooth-assisted electrochemical sensor for the analysis of triclosan (TCS), which is a broad-spectrum antibacterial and antifungal compound. Initially, a laboratory-fabricated screen-printed electrode (LabSPE) was constructed by depositing various conductive inks (carbon and silver) onto a polycarbonate substrate surface using the screen-printing technique. Subsequently, the surface of the working electrode on the LabSPE was modified with a composite comprising Ni-doped Ag2S (Ni-Ag2S) and chitosan (Chit), rendering it suitable for the detection of TCS. The effect of Ni-Ag2S amount, Chit amount, composite film thickness, and pH of buffer solution on the electrochemical signal of the prepared sensor (Ni-Ag2S-Chit/LabSPE) was also optimized. The sensor displayed remarkable analytical performance in the range of 0.05 nM-90 μM TCS with a low detection limit of 0.02 nM. The sensitivities of Ag2S/LabSPE and Ni-Ag2S-Chit/LabSPE were found to be 0.0838 µAµM−1 and 0.1097 µAµM−1, respectively, proving the contribution of doped Ni to the performance of the present sensor. The selectivity, reproducibility, reproducibility, reproducibility and stability of the sensor as well as its practical applicability have been successfully in two commercial products.