The genus Gundelia is currently represented with 18 species, of which 16 are in Turkey. In genus Gundelia, the chromosomal data were reported from 12 species. In the present study, it is aimed to eliminate the deficiencies in the knowledge about chromosomal data of Gundelia species. In Genus Gundelia, only a single chromosome number had been detected as 2n=18 so far. The chromosome numbers of four species were reported here for the first time: G. armeniaca, G. cappadocica, G. siirtica, and G. tehranica. In addition, the polyploidy in the genus was rare and G. anatolica was identified as the first polyploid species. All karyotypes except G. tehranica were symmetrical, consisting of metacentric and submetacentric chromosomes. Secondary constrictions were observed in the distal regions of the long arms of the longest metacentric and submetacentric chromosomes. Thus, the chromosomal data of all Turkish Gundelia species were completed. In conclusion, the present study presented new data into the karyological records relating the karyotype evolution and interspecific relations of genus Gundelia.