Objective: The family Lamiaceae, consists of medicinal and aromatic plants, is represented by 46 genera and 571 species in Turkey. Traditional uses for Calamintha Miller species include utilizing as a spice, stimulant, antispasmodic, diaphoretic, expectorant, as well as treatment for stomach and throat aches. C. pamphylica subsp. davisii (Contandr. & Quézel) P.H. Davis is accepted as synonym of Clinopodium pamphylicum subsp. davisii (Contandr. & Quézel) Govaerts. C. pamphylicum subsp. davisii is an endemic species to Turkey. Until now, no biological and phytochemical research has been reported on this plant except for its essential oil research. Examining the antioxidant, and enzyme inhibition effects of C. pamphylicum subsp. davisii, as well as determining its phytochemical composition were the targets of the current study. Material and Method: To evaluate in vitro antioxidant potential (DPPH, ABTS, iron chelating, total phenol and flavonoid amounts) and enzyme inhibitory activities, such as acetylcholinesterase, butyrylcholinesterase, α-glucosidase, α-amylase and tyrosinase for C. pamphylicum subsp. davisii were measured with spectrophotometric methods. The quantities of different phenolic acids and flavonoids were measured using HPLC to analyze the phenolic composition of the plant extracts. Result and Discussion: Our results indicated that except for iron chelating methanol extract exhibited higher antioxidant properties over water extract using DPPH, and ABTS methods. In addition, methanol extract displayed more inhibition on the tested enzymes except for acetylcholinesterase, and α-glucosidase than water extract. As for HPLC findings, although chlorogenic acid was signified as the main phenolic compound for the methanol extract, caffeic acid was specified as the major phenolic for the water extract. These results provide a scientific basis for C. pamphylica subsp. davisii, which possessed the traditional uses in Turkish folk medicine.