In this study, it was aimed to determine the effects of colors and the usage rates in interior spaces on the spatial perceptions of users. With this objective, a living space was designed in a manner that would have three different colors (warm, neutral, and cool) and usage rates at 10%, 50%, and 100% and digital visuals were prepared. A total of 233 subjects participated in the research questionnaire and the data obtained were analyzed. In conclusion, it was determined that the living spaces which were colored at a low rate were perceived more positively according to the Semantic Differentiation Scales compared to the completely colored spaces. The spaces where warm and cool colors were used at the rate of 10% were perceived as more spacious and beautiful compared to the spaces where colors were used at the rates of 50% and 100%, whereas it was observed that when the values received were from positive to negative, they were listed in the form of 10% > 50% > 100%. Despite this, it was determined that the spaces where warm colors were used at the rates of 50% and 100% were perceived as more informal. Furthermore, it was observed that the differences in the colors used on the walls and the usage rates were changing according to gender and professions. Accordingly, it was determined that especially females and interior architects preferred, even more, the spaces with warm colors having a low usage rate.