Within the statistical multifragmentation model we study modifications of the surface and symmetry energy of primary fragments in the freeze-out volume. The ALADIN experimental data on multifragmentation obtained in reactions induced by high-energy projectiles with different neutron richness are analyzed. We have extracted the isospin dependence of the surface energy coefficient at different degrees of fragmentation. We conclude that the surface energy of hot fragments produced in multifragmentation reactions differs from the values extracted for isolated nuclei at low excitation. At high fragment multiplicity, it becomes nearly independent of the neutron content of the fragments. © 2006 The American Physical Society.