Marbofloxacin (MBX) is a 3rd generation fluoroquinolone specifically developed for animal health and is an approved antimicrobial agent for the cure of mastitis and respiratory diseases in cattle and swine. Ketoprofen (KTP) is a nonsteroidal anti-inflammatory drugs (NSAID) belonging to the aryl propionic acid group and is used in musculoskeletal inflammation and pain, abdominal pain and other inflammatory circumstances. The objective of this study is to investigate the impact of simultaneous administration of MBX and KTP on the coagulation and biochemical parameters in calves. In the study, 18 clinically healthy calves were randomly separated into 3 groups of six animals each. The first group of calves received a single dose of MBX at 8 mg/kg, the second group received MBX at 8 mg/kg along with concurrent KTP at 3 mg/kg and the third group received only KTP at 3 mg/kg dose. The drugs were administered via intramuscular (IM) injection in the neck region. Biochemical and coagulation parameters were evaluated using an automated analyzer and coagulation analyzer, respectively. The IM injection of MBX into calves significantly increased creatine kinase (CK) and lactate dehydrogenase (LDH) values, which may be related to muscle damage. This may limit the administration via IM route of MBX to calves. KTP increased the prothrombin time (PT) and activated partial thromboplastin time (aPTT) values but decreased the fibrinogen value. It can be stated that in alone MBX, and KTP administration and their combination, further investigations are required to determine the safety of drugs after repeated administrations and other administration routes in calves.