Aim: This study is aimed to determine the validity and reliability of the Turkish adaptation of the HSOPSC 2.0 for Turkish hospitals. Methods: This two-stage psychometric study was conducted with 613 nurses (The response rate of the nurses participated in the study is 72.11%.) at a university hospital between July 2021 and February 2022. In the first stage, the adaptation of the scale was performed. Construct validity was determined at the second stage using confirmatory factor analysis. Reliability was tested using the internal consistency coefficient. Results: The adaptation results showed that the Turkish version of the scale was adequate for language and content validation. This scale, consisting of 32 items and ten subscales, showed a significantly good fit with the original scale according to confirmatory factor analysis. The Cronbach's alpha coefficient for the subscales ranged between 0.72 and 0.82. Conclusions: The Turkish version of the Hospital Survey on Patient Safety Culture consistently showed acceptable psychometric reliability and validity characteristics.