Introduction and hypothesis: To translate the Prolapse and Incontinence Knowledge Questionnaire (PIKQ) into Turkish and test its validity and reliability. Methods: The study included 341 women. The translation of the PIKQ, which comprised of the urinary incontinence (PIKQ-UI) and pelvic organ prolapse (PIKQ-POP) sections, was performed in accordance with international recommendations. The Incontinence Quiz (IQ) and the Visual Analog Scale (VAS) were applied to assess the level of knowledge about POP and UI. Psychometric analyses consisted of assessing the following: (1) construct validity by confirmatory factor analysis, (2) criterion and known group validity, (3) internal consistency reliability by the KR-20 coefficient, and (4) test-retest reliability over 1 week with the intraclass correlation coefficient (ICC). Results: All fit indices except the Standardized Root Mean Square Residual indicated acceptable fit for the final models. Criterion validity was supported by moderate correlations between the PIKQ-UI and the IQ (rho = 0.679, p < 0.001). There were positive and weak linear correlations between the PIKQ-UI and PIKQ-POP scores and their corresponding perceived knowledge scores (rho = 0.351, p = 0.013 and rho = 0.345, p = 0.014, respectively). The known group validity did not show differences indicating that participants did not have enough knowledge about UI and/or POP even when they had the condition or acquaintance with them (p = 0.852 and p = 0.185, respectively). Reliability was excellent as indicated by the ICCs of 0.91–0.90, and KR-20 of 0.67–0.75 indicated good internal consistency for the PIKQ-UI and PIKQ-POP, respectively. Conclusions: The Turkish version of the PIKQ is a valid and reliable instrument to assess the knowledge of UI and POP.