This study was carried out to investigate the presence of BNoV in cattle and its phylogenetic analysis. The fecal samples from a total of 80 cattle (ages 2 to 5 years old) brought to a slaughterhouse in Konya for slaughter (between 2019-2020) were collected, regardless of clinical symptoms and gender, and examined for the presence of nucleic acid by RT-PCR. Six samples out of the tested fecal samples (7.5%) were found to be positive for the presence of BNoV nucleic acid. DNA sequences were analyzed by comparing the nucleotide gene sequences specific to BNoV isolates with GIII genotype and the RdRp gene region of the virus in different regions of Turkey and the world. As a result of the phylogenetic analysis in line with the data obtained, it was determined that the virus genotype circulating in the sampling region was included in the GIII.2 cluster-2. It can be concluded that the BnoV infection exists in the country, therefore, it can be stated that protection and control measures should be applied within the scope of the struggle.