The essential oil obtained from Lavandula angustifolia Mill. (Lavander) has many pharmacological effects, including antioxidant, antifungal, antibacterial and carminative effects. Lavandula angustifolia essential oil (LEO) contains monoterpene compounds, mainly linalool and linalyl acetate and various phenolic acids such as caffeic acid, vanillic acid, ferulic acid and chlorogenic acid. The hydrodistillation of the aerial parts of L. angustifolia produced a pale yellow oil. Gas chromatography-Gas chromatography/Massspectrometry (GC-GC/MS) results showed that LEO contains monoterpene alcohols that primarily include linalool (35.91%), 4-Terpineol (6.10%), α-Terpineol (4.49%) and lavandulol (2.49%). As a result of the cytotoxicity analysis of LEO, the IC50 value was found to be 0.372 mg/mL. LEO was found to be non-mutagenic against the bacterial test strain Salmonella typhimurium TA 98 with S9 fraction while the highest concentration (9.58 mg/mL) was found to be either mutagenic or cytotoxic against TA 98 without S9 fraction. The essential oil’s antioxidant capacity was found 6.522±0.069 mg/mL while the total phenolic content was 1.22±0.04 mg GAE/g extract (Gallic acid equivalent/g extract). Anti-inflammatory activity of LEO was 1.238± 0.026 mg/mL while that of indomethacine was 0.022±0.003 mg/mL. In conclusion, when the results are evaluated in terms of in vitro biological activities, LEO can be considered as a potential herbal product with active compounds that should be examined in future studies.