Onopordum spp. is investigated experimentally for biofuel properties. The plant is introduced, biofuel production processes are explained, and exergy destruction is quantified. The aim of the research is to provide a solid candidate for production and adaptation of drought-resistant renewable energy plants. The plant is not regarded as a probable source of agricultural food ethics and insignificant food properties. The onopordum plant is regarded as an ideal biofuel plant. Solid fuels in pellet form were produced from its stalk and pulp, while biodiesel was produced from its oil seeds by means of a patented method. Properties of the biodiesel that was produced from the oil of the onopordum were determined. An exergy analysis was conducted in order to find the destructed exergy by the biofuel production process. The exergy analysis is crucial to distinguish similar energy plants with similar energy contents. The onopordum pellets have lower heating value of about 15.10 MJ/kg while liquid products have values of about 40 MJ/kg. The irreversibility throughout the biofuel production process was found to be 126.692 MJ. According to the obtained quantities and the nature of the plant, the onopordum spp. has a great potential for energy plant cultivation applications.