The Mycobiota of Samanlı Mountains were investigated in this study. Specimens were collected during 3 years between November 2012 and November 2015. 510 macrofungal taxa belonging to 197 genera within 84 families were recorded with field and laboratory studies. Of these, 37 genera and 57 taxa belong to Ascomycota, while 160 genera and 453 taxa belong to Basidiomycota. Nine species were found for the first time in Turkey from Basidiomycota. These taxa are Amanita subnudipes (Romagn.) Tulloss, Hebeloma quercetorum Quadr., Hygrocybe obrussea (Fr.) Wunsche, Lactarius mediterraneensis Llistosella & Bellù, Lactifluus glaucescens (Crossl.) Verbeken, Russula lilacea Quél., R. rubra (Lam.) Fr., Stereopsis reidii Losi & A. Gennari and Tricholoma roseoacerbum A. Riva. The Stereopsidaceae family and the genus Stereopsis D.A. Reid was found for the first time in Turkey. The richest families in terms of the number of taxa are Russulacea with 58 taxa (11.3%), Agaricaceae with 46 taxa (8.9%), Tricholomataceae with 43 taxa (8.4%), Boletaceae with 32 taxa (6.2%), Polyporaceae with 23 taxa (4.5%) and the most crowded genera are Russula Pers. with 41 taxa (8%), Tricholoma (Fr.) Staude with 26 taxa (5%), Amanita Dill. ex Boehm. with 19 taxa (3.7%), Lactarius Pers. with 16 taxa (3.1%) and Inocybe (Fr.) Fr. with 14 taxa (2.7%). The ecological status of the species is as follows; 245 (48%) are saprobe, 226 (45%) are mycorrhizal, 20 (3.7%) are lignicolous, 18 (2.9%) are parasite, and one is entomopathogenic. Habitat distribution in the research area is as follows: 300 species in Abies nordmanniana (Stev.) Spach. subsp. bornmuelleriana (Mattf.) Coode & Cullen forest, 295 species in Fagus orientalis Lipsky forest, 125 species in Quercus spp. forest, 88 species in Pinus nigra J.F.Arnold forest, 56 species in Castanea sativa Mill. forest, 53 species in Pinus sylvestris L. forest, 49 species in Carpinus orientalis Mill. forest and 24 species in Pinus maritima Lam. forest.