Background: The aim of this study is to evaluate salivary and gingival crevicular fluid(GCF) levels of Developmental Endothelial Locus-1(Del-1) and Lymphocyte function-associated antigen-1(LFA-1) which are regulators of firm adhesion of neutrophils in neutrophil recruitment in different grades of periodontitis. Methods: A total of 42 non-smoker and healthy individuals who applied to Selcuk University, Faculty of Dentistry, Department of Periodontology were included in this study. After clinical and radiographical examination, patients were grouped as Grade B(n=23) or Grade C(n=19). Probing pocket depth(PPD), clinical attachment level(CAL), plaque(PI) and, gingival index(GI) and bleeding sites on probing(BOP) were recorded. Saliva and GCF samples were collected and cytokine analysis were performed by ELISA. Mann-Whitney U test is used for statistical analysis and level of significance was determined as p≤0.05. Results: All clinical parameters were found higher in Grade C periodontitis group, while CAL and GI values showed no significance.In biochemical analysis, there was no significance between groups in salivary and GCF levels of LFA-1 and Del-1.Besides these findings;there was a tendency to be higher in salivary and GCF levels of LFA-1 in Grade C group.As an antagonist mediator of LFA-1, salivary Del-1 tends to be higher in Grade B periodontitis group while GCF levels of Del-1 tends to be higher in Grade C periodontitis group. Conclusion: Findings of this study suggested that salivary and GCF level of Del-1 and LFA-1 might give information about disease severity.Further longitudinal studies with greater numbers of participants are required to define the roles of these cytokines in periodontal pathogenesis.