It was aimed in this study to determine the evaluations for “spatial quality,” “social adaptation,” and “individual productivity” of students in design studios, which used different light color temperature values. With this intention, a detailed research questionnaire was developed for measuring the effects on spatial perception of low color temperatures, daylight, and high color temperatures for a total of three different light color temperature values. A total of 113 students participated in the research questionnaire and the data obtained were analyzed with the SPSS package program. In conclusion, it was determined that the spaces lighted with the low color temperatures and daylight had more positive effects on the perceptual evaluations of the students compared to the spaces lighted with the high color temperatures according to the “spatial quality,” “social adaptation,” and “individual productivity” scales. Another result was that it was observed that the low color temperatures had somewhat more positive perceptual values compared to daylight. In addition, it was determined that males perceived more positively compared to females the interior design characteristics of the design studios.