Purpose: This study was conducted to evaluate the effect of high intensity interval training (HIIT) on macular microcirculation, measured by swept source optical coherence tomography angiography (ss OCTA) in young football players. Methods: Football players between 18-20 years old were included. After a detailed ophthalmological examination, physiological parameters, including height, body weight, body fat, systemic blood pressure, hematocrit values, oxygen saturation, and heart rate, were recorded. Intraocular pressure and ss OCTA parameters were measured one day before and the day after the high intensity interval training program using DRI OCT Triton (Topcon, Tokyo, Japan) between 11:00 am and 1:00 pm. Results: Fifteen participants completed the study. All were males with a mean age of 18.1 ± 0.4 years. Systolic and diastolic blood pressure and oxygen saturation did not change significantly (P > 0.05), while hematocrit levels increased remarkably (P = 0.049) after the HIIT program. Heart rates and intraocular pressure decreased (P = 0.003, P = 0.017, respectively). There was a significant increase in the central vessel density in deep capillary plexus (before: 18.7 ± 3.8%, after: 21.1 ± 4.5%) and central vessel density in choriocapillaris (before: 54.5 ± 2.8%, after 56.9 ± 2.2%) (P = 0.02, P = 0.02, respectively), although no changes were observed in other ss OCTA or in the central macular thickness and subfoveal choroidal thickness. Conclusion: A 6 week, high intensity interval training program with three exercise sessions per week seems not to alter mean superficial vascular densities, deep foveal avascular zone, and superficial foveal avascular zones, central macular thickness, or subfoveal choroidal thickness, while the central deep vascular density and central choriocapillaris vascular density increased remarkably among ss OCTA parameters.