Objective: The aim of this study was to compare the total protein content in 24 hours urine with dipstic, protein / creatinine (P / K) ratio in spot urine in our pregnant women who were diagnosed with preeclampsia. Material and Methods: In this study, 317 patients who underwent dipstick method, P / K ratio and total protein analysis in 24-hour urine were included in the study with a prediagnosis of preeclampsia at a university hospital gynecology and obstetrics clinic between January 2016 and December 2018. These three values of patients were compared with each other. Results: When the total proteinuria threshold in the 24-hour urine was 300 mg / day, the P-C ratio cut-off value was 0.34 mg protein / mg creatinine, the sensitivity was 68.9% and the specificity was 78.7%. A moderate, significant correlation was observed between total urinary P / K ratio and total proteinuria in 24-h urine (r= 0.629 p <0.001). Conclusion: It can be used as an alternative test in the 24-hour urine total protein test of P / C ratio in spot urine in emergencies where there is not enough time for 24 hour total urine analysis.