In recent years, plant breeding of some cultivars such as sumac has attracted more attention because their contributions to human health started to be revealed. Turkey being its origin, sumac (Rhus coriaria L.) is widely used as a medicinal plant and spice. However, the sporadic cultivation of cultivars such as sumac endangers its existence and future. Therefore, the protection of this cultivar and its genetic characterization as well as encouraging the cultivation of this cultivar through plant breeding is of utmost importance. In this respect, this study aims to determine the genetic diversities among 24 different sumac (Rhus coriaria L.) genotypes. 17 different SRAP (sequence related amplified polymorphism) and 12 ISSR (inter simple sequence repeat) primers were used in this study, and, as a result, 133 amplifications were obtained. 130 of these amplifications were polyphormic, the average value of polymorphism were determined as 97.74%. In addition, genetic difference among the genotypes were calculated, which yielded a percentage of 45%. The highest difference was observed between S19 and S5 (73%), while the lowest genetic difference was found between S23 and S24 genotypes (20%). These results suggest that sumac genotypes displaying high genetic difference will be selected as parents in the plant breeding studies on sumac cultivars in the future, thus increasing variation rate.