Satureja cilicica P.H. Davis is an endemic species of Lamiaceae, distributed in Turkey. In this study, the essential oil of S. cilicica was obtained by hydrodistillation from the aerial parts. The essential oil was analyzed by GC/MS and the main constituents are identified. Radical scavenging capacities of oil obtained from S. cilicica were determined by using DPPH and ABTS radical scavenging assays. In addition, ferric-reducing power of the essential oil was detected. Cytotoxic activity of the essential oil was determined by MTT assay after 24 h treatment of MCF-7. The major components of the essential oil are p-cymene (17.68%), carvacrol (14.02%), γ-terpinene (11.23 %) and thymol (8.76%). Radical scavenging capacities of the essential oil were measured as 3.28±0.02 and 238.15±3.59 mg Trolox equivalent per milliliter of oil in term of DPPH and ABTS radical scavenging, respectively. Ferric-reducing power of essential oil was found 39.76±3.66 mg Trolox equivalent per milliliter of essential oil. Growth inhibition effect of essential oil against MCF-7 cancer cell is established. According to the results, essential oil of S. cilicica exhibit low cytotoxic activity, with IC50 values, with IC50 value of 268 µg/mL. The findings of the analyzed species are compared with the results of previous studies.